Microtubules regulate brush border formation

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dc.citation.titleJournal of Cellular Physiology
dc.citation.volume233
dc.contributor.orcidhttps://orcid.org/0000-0002-6967-5431
dc.creatorTonucci, Facundo Mauro
dc.creatorFerretti, Anabela Cecilia
dc.creatorAlmada, Evangelina
dc.creatorCribb, Pamela
dc.creatorVena, Rodrigo
dc.creatorHidalgo, Florencia
dc.creatorFavre, Cristian
dc.creatorTyska, Matt J.
dc.creatorKaverina, Irina
dc.creatorLarocca, María Cecilia
dc.date.accessioned2025-02-25T13:54:26Z
dc.date.available2025-02-25T13:54:26Z
dc.date.issued2017-11-06
dc.description.abstractMost epithelial cells contain apical membrane structures associated to bundles of actin filaments, which constitute the brush border. Whereas microtubule participation in the maintenance of the brush border identity has been characterized, their contribution to de novo microvilli organization remained elusive. Hereby, using a cell model of individual enterocyte polarization, we found that nocodazole induced microtubule depolymerization prevented the de novo brush border formation. Microtubule participation in brush border actin organization was confirmed in polarized kidney tubule MDCK cells. We also found that centrosome, but not Golgi derived microtubules, were essential for the initial stages of brush border development. During this process, microtubule plus ends acquired an early asymmetric orientation toward the apical membrane, which clearly differs from their predominant basal orientation in mature epithelia. In addition, overexpression of the microtubule plus ends associated protein CLIP170, which regulate actin nucleation in different cell contexts, facilitated brush border formation. In combination, the present results support the participation of centrosomal microtubule plus ends in the activation of the polarized actin organization associated to brush border formation, unveiling a novel mechanism of microtubule regulation of epithelial polarity.
dc.description.filFil: Tonucci, Facundo Mauro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Ferretti, Anabela Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Almada, Evangelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Cribb, Pamela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Vena, Rodrigo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Hidalgo, Florencia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Favre, Cristian. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.filFil: Tyska, Matt J. Vanderbilt University. School of Medicine. Vanderbilt University Medical Center. Vanderbilt-Ingram Cancer Center; United States.
dc.description.filFil: Tyska, Matt J. Vanderbilt University. School of Medicine. Vanderbilt University Medical Center. Epithelial Biology Center. Department of Cell and Developmental Biology; United States.
dc.description.filFil: Tyska, Matt J. U.S. Department of Veterans Affairs. Nashville VA Medical Center; United States.
dc.description.filFil: Kaverina, Irina. Vanderbilt University. School of Medicine. Vanderbilt University Medical Center. Vanderbilt-Ingram Cancer Center; United States.
dc.description.filFil: Kaverina, Irina. Vanderbilt University. School of Medicine. Vanderbilt University Medical Center. Epithelial Biology Center. Department of Cell and Developmental Biology; United States.
dc.description.filFil: Kaverina, Irina. U.S. Department of Veterans Affairs. Nashville VA Medical Center; United States.
dc.description.filFil: Larocca, María Cecilia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Fisiología Experimental (IFISE-CONICET); Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP0287
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT2012-2198
dc.description.sponsorshipGobierno de la República Argentina: Bec.Ar
dc.description.sponsorshipNational Institutes of Health (NIH): R01-GM078373, R01-DK106228, R01-DK075555, R01-DK095811
dc.description.versionpeerreviewed
dc.format.extent1-13
dc.identifier.citationTonucci, F. M., Ferretti, A. C., Almada, E., Cribb, P., Vena, R., Hidalgo, F., Favre, C., Tyska, M. J., Kaverina, I. and Larocca, M. C. (2017). Microtubules regulate brush border formation. Journal of Cellular Physiology, 233(2), 1468-1480. https://doi.org/10.1002/jcp.26033
dc.identifier.e-issn1097-4652
dc.identifier.issn0021-9541
dc.identifier.urihttps://hdl.handle.net/2133/28933
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1002/jcp.26033
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1002/jcp.26033
dc.rightsopenAccess
dc.rights.holderTonucci, Facundo Mauro
dc.rights.holderFerretti, Anabela Cecilia
dc.rights.holderAlmada, Evangelina
dc.rights.holderCribb, Pamela
dc.rights.holderVena, Rodrigo
dc.rights.holderHidalgo, Florencia
dc.rights.holderFavre, Cristian
dc.rights.holderTyska, Matt J.
dc.rights.holderKaverina, Irina
dc.rights.holderLarocca, María Cecilia
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectActin
dc.subjectBrush border
dc.subjectEpithelial polarity
dc.subjectMicrotubules
dc.subjectMTOC
dc.subjectMicrovilli
dc.titleMicrotubules regulate brush border formation
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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