Alterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvae

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dc.citation.titleJournal of Helminthologyes
dc.citation.volume94es
dc.creatorPonce de León, Patricia
dc.creatorBellini, Mariana
dc.creatorCastellini, Horacio V.
dc.creatorRiquelme, Bibiana Doris
dc.date.accessioned2020-03-02T22:04:17Z
dc.date.available2020-03-02T22:04:17Z
dc.date.issued2018-11-29
dc.descriptionThe complex life cycle of Trichinella spiralis includes the migration of newborn larvae through the bloodstream to their encystment in muscle. The parasite establishes an intimate contact with the erythrocytes of the host both during the migration of the newborn larvae and when encysting, as this parasite causes intense vascularization in the muscle cell. The goal of this work was to study the effects of various concentrations of T. spiralis muscle larvae (ML) on erythrocyte membranes. The treatment was performed by incubating human erythrocytes with equal volume of different concentrations of ML for 30 minutes, with controlled agitation (37°C). The control erythrocytes (with no contact with the larvae) were incubated in the same way with an equal volume of physiological solution. To evaluate the alterations to the erythrocytes by the action of the larvae and in the respective controls, an Erythrocyte Rheometer and a Digital Image Analysis technique were used. The results indicated that when the larval concentration was higher, the aggregation and erythrocyte membrane alterations were also higher. Also, the erythrocyte deformability index and the erythrocyte elasticity increased. The values of isolated cell coefficient varied from 0.51 in the treatment with 100 larvae/ml to 0.91 in the incubation with 1000 larvae/ml. This experiment shows that T. spiralis muscle larvae affect significantly the red blood cell aggregation and the erythrocyte viscoelastic properties.es
dc.description.filFil: Ponce de León, Patricia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Bellini, Mariana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Castellini, Horacio V. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.es
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.sponsorshipUniversidad Nacional de Rosarioes
dc.formatapplication/pdf
dc.format.extentE17es
dc.identifier.issn1475-2697es
dc.identifier.urihttp://hdl.handle.net/2133/17697
dc.language.isoenges
dc.publisherCambridge University Presses
dc.relation.publisherversionhttps://www.cambridge.org/core/journals/journal-of-helminthology/article/alterations-of-the-erythrocyte-membrane-by-in-vitro-action-of-trichinella-spiralis-muscle-larvae/B8DC0F8CE1FB5C814266CBC1B93699AA#fndtn-informationes
dc.relation.publisherversionhttp://dx.doi.org/10.1017/S0022149X18001062es
dc.rightsembargoedAccesses
dc.rights.holderCambridge University Presses
dc.rights.holderPonce de León, Patriciaes
dc.rights.holderBellini, Marianaes
dc.rights.holderCastellini, Horacio V.es
dc.rights.holderRiquelme, Bibiana Dorises
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticases
dc.rights.textAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.eses
dc.subjectErythrocyte aggregationes
dc.subjectErythrocyte viscoelasticityes
dc.subjectTrichinella spiralis muscle larvaees
dc.titleAlterations of the erythrocyte membrane by in vitro action of Trichinella spiralis muscle larvaees
dc.typearticle
dc.typeartículo
dc.typeacceptedVersion
dc.type.collectionarticulo
dc.type.versionacceptedVersiones

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(FBIOyF) Departamento de Química Física - Artículo de Revista
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