Assessing different ways of Bacillus subtilis spreading over abiotic surfaces

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dc.citation.titleBio-protocol journal
dc.citation.volume9
dc.creatorBartolini, Marco
dc.creatorGrau, Roberto Ricardo
dc.date.accessioned2024-10-25T14:41:59Z
dc.date.available2024-10-25T14:41:59Z
dc.date.issued2019-11-20
dc.description.abstractSurface-associate motility on biotic and abiotic environments is a key mechanism used by the model bacterium Bacillus subtilis and its closest relatives (i.e., B. amyloliquefaciens, B. thuringiensis, B. cereus, B. pumilus) for surface colonization and spreading across surfaces. The study of this mechanism in a research, industrial or clinic laboratory is essential; however, precautions should be taken for the reproducibility of the results, for example, the procedure to inoculate the bacteria on the testing plate, the humidity of the plate and the agar concentration. In this protocol, we describe, using Bacillus subtilis, how to perform these assays and, in addition, we show how by varying the agar concentration in the plate, you can make a first approximation of what type of motility has other bacterial species.
dc.description.filFil: Bartolini, Marco. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología (CONICET); Argentina.
dc.description.filFil: Grau, Roberto Ricardo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología (CONICET); Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipFondo para la Investigación Científica y Tecnológica (FONCyT)
dc.description.sponsorshipFulbright Program
dc.description.sponsorshipPew Research Center
dc.description.versionpeerreviewed
dc.format.extent1-10
dc.identifier.citationBartolini, M. and Grau, R. (2019). Assessing Different Ways of Bacillus subtilis Spreading over Abiotic Surfaces. Bio-protocol journal, 9(22). https://doi.org/10.21769/bioprotoc.3425
dc.identifier.issn2331-8325
dc.identifier.urihttps://hdl.handle.net/2133/27985
dc.language.isoen
dc.publisherBio-protocol L.L.C.
dc.relation.publisherversionhttps://doi.org/10.21769/bioprotoc.3425
dc.relation.publisherversionhttps://bio-protocol.org/en/bpdetail?id=3425&type=0
dc.rightsopenAccess
dc.rights.holderBartolini, Marco
dc.rights.holderGrau, Roberto Ricardo
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBacillus
dc.subjectSpreading
dc.subjectSwimming
dc.subjectSwarming
dc.subjectSliding
dc.subjectTwitching
dc.subjectSurface-colonization
dc.subjectBacillus subtilis
dc.subjectBacteria
dc.titleAssessing different ways of Bacillus subtilis spreading over abiotic surfaces
dc.typearticulo
dc.type.versionpublishedVersion

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