Effect of zinc on boar sperm liquid storage

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dc.citation.titleFrontiers in Veterinary Science
dc.citation.volume10
dc.creatorMarini, Patricia Estela
dc.creatorFernández Beato, Luciana
dc.creatorCane, Fernando
dc.creatorTeijeiro, Juan Manuel
dc.date.accessioned2024-12-06T15:57:36Z
dc.date.available2024-12-06T15:57:36Z
dc.date.issued2023-02-02
dc.description.abstractStorage and transport of liquid boar sperm for artificial insemination (AI) requires the addition of solutions called extenders, which increase the volume of the ejaculate and help preserve its functional characteristics. Yet, the quality of sperm decreases over time primarily due to the increased production of reactive oxygen species (ROS) that damage the plasma membrane. Many commercial extenders are supplemented with additives that mitigate this eect. In semen, zinc is supplied at high concentration on the seminal plasma and helps protect the plasma membrane of sperm. However, zinc in the seminal plasma is diluted and chelated upon addition of extenders for storage, potentially reducing its antioxidant eect. Here we characterize viability, motility, mitochondrial activity, DNA integrity and ROS content of boar sperm diluted with Sus (Medi Nova, Italy) extender supplemented with dierent concentrations of ZnCl2, at intervals after dilution during 3 days. The ability of sperm supplemented with 2 mM ZnCl2 to fertilize oocytes in vivo of was also tested. Sperm viability was over 82% for all treatments. Mitochondrial integrity analysis, measured by Cytochrome c activity, indicated a protector eect of Zn, noted as a reduced number of sperm with extensive loss of mitochondrial activity. Acrosomal integrity was improved by treatment with all concentrations of ZnCl2 tested. Sperm kinematics were aected by treatment with ZnCl2, showing higher percentage of progressive and rapid sperm in doses supplemented with 2mM ZnCl2. ROS levels and chromatin integrity did not show dierences between ZnCl2-supplemented doses and the control. Fertilization rate, total number, live, still born and mummified piglets did not change when sperm were diluted with extender containing 2 mM ZnCl2. The presented characterization indicates that Zn addition to Sus extender have a protective eect on mitochondrial sheath and acrosomal membranes; and provides the basis for further studies aimed to optimize sperm performance in AI.
dc.description.filFil: Marini, Patricia Estela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.filFil: Marini, Patricia Estela. Consejo de Investigaciones de la Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Fernández Beato, Luciana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.filFil: Cane, Fernando. Medax; Argentina.
dc.description.filFil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.filFil: Teijeiro, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.sponsorshipUniversidad Nacional de Rosario
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (FONCyT): PICT 2017-1006
dc.format.extent01-11
dc.identifier.citationMarini, Patricia Estela; Fernández Beato, Luciana; Cane, Fernando; Teijeiro, Juan Manuel; Effect of zinc on boar sperm liquid storage; Frontiers Media; Frontiers in Veterinary Science; 10; 2-2023; 1-11
dc.identifier.issn2297-1769
dc.identifier.urihttps://hdl.handle.net/2133/28330
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://doi.org/10.3389/fvets.2023.1107929
dc.relation.publisherversionhttps://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1107929/full
dc.rightsopenAccess
dc.rights.holderMarini, Patricia Estela
dc.rights.holderFernández Beato, Luciana
dc.rights.holderCane, Fernando
dc.rights.holderTeijeiro, Juan Manuel
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPorcine
dc.subjectSpermatozoa
dc.subjectZinc
dc.subjectExtender
dc.subjectArtificial insemination
dc.titleEffect of zinc on boar sperm liquid storage
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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