Assessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols

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dc.citation.titleBMC Infectious Diseases
dc.citation.volume20
dc.contributor.orcidhttps://orcid.org/0000-0003-4925-9075
dc.creatorBolatti, Elisa María
dc.creatorHošnjak, Lea
dc.creatorChouhy, Diego
dc.creatorCasal, Pablo E.
dc.creatorRe Louhau, María F.
dc.creatorBottai, Hebe
dc.creatorKomloš , Kristina Fujs
dc.creatorPoljak, Mario
dc.creatorGiri, Adriana Angélica
dc.date.accessioned2024-05-07T16:20:39Z
dc.date.available2024-05-07T16:20:39Z
dc.date.issued2020-04-07
dc.description.abstractBackground: Human papillomaviruses (HPVs) have been divided into mucosal and cutaneous types according to their primary epithelial tissue tropism. However, recent studies showed the presence of several cutaneous types in mucosal lesions and healthy mucosa from different anatomical sites. Methods: Here, the HPV prevalence and type-specific distribution were assessed in a variety of mucosal samples from 435 individuals using a combination of two established broad-spectrum primer systems: Gamma-PV PCR and CUT PCR. Results: Overall HPV prevalence in anal canal swabs, cervical cancer biopsies, genital warts and oral swabs was 85, 47, 62 and 4%, respectively. In anal canal swabs, Alpha-PVs were most frequently found (59%), followed by Gamma-(37%) and Beta-PVs (4%). The prevalence and persistence of HPV infection in the anal canal of 226 individuals were further explored. Overall HPV, Gamma-PVs and multiple HPV infections were significantly higher in men vs. women (p = 0.034, p = 0.027 and p = 0.003, respectively); multiple HPV infections were more common in individuals ≤40 years (p = 0.05), and significantly higher prevalence of Gamma-PVs and multiple HPV infections was observed in HIV-1-positive vs. HIV-1-negative individuals (p = 0.003 and p = 0.04, respectively). Out of 21 patients with follow-up anal swabs, only one persistent infection with the same type (HPV58) was detected. Conclusions: Our findings suggest that Gamma-PVs (except species Gamma-6) are ubiquitous viruses with dual muco-cutaneous tissue tropism. Anal canal Gamma-PV infections may be associated with sexual behavior and the host immune status. This study expands the knowledge on Gamma-PVs’ tissue tropism, providing valuable data on the characteristics of HPV infection in the anal canal.
dc.description.filFil: Bolatti, Elisa María. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Grupo Virología Humana (CONICET-IBR); Argentina.
dc.description.filFil: Bolatti, Elisa María. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Hošnjak, Lea. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Chouhy, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Grupo Virología Humana (CONICET-IBR); Argentina.
dc.description.filFil: Chouhy, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Casal, Pablo E. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Re Louhau, María F. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Grupo Virología Humana (CONICET-IBR); Argentina.
dc.description.filFil: Bottai, Hebe. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Estadística y Procesamiento de Datos; Argentina.
dc.description.filFil: Komloš, Kristina Fujs. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Poljak, Mario. University of Ljubljana. Faculty of Medicine. Institute of Microbiology and Immunology; Slovenia.
dc.description.filFil: Giri, Adriana Angélica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario. Grupo Virología Humana (CONICET-IBR); Argentina.
dc.description.filFil: Giri, Adriana Angélica. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT-2012-0652
dc.description.sponsorshipJavna Agencija za Raziskovalno Dejavnost RS: P3-0083
dc.description.sponsorshipEuropean Society of Clinical Microbiology and Infectious Diseases: 1146
dc.description.sponsorshipBilateral Cooperation Program between the Republic of Argentina and Republic of Slovenia: AR/14/05, BI-AR/15-17-005
dc.format.extent1-10
dc.identifier.issn1471-2334
dc.identifier.urihttps://hdl.handle.net/2133/26999
dc.language.isoen
dc.publisherBMC
dc.relation.publisherversionhttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-4893-3
dc.relation.publisherversionhttps://doi.org/10.1186/s12879-020-4893-3
dc.rightsopenAccess
dc.rights.holderBolatti, Elisa María
dc.rights.holderHošnjak, Lea
dc.rights.holderChouhy, Diego
dc.rights.holderCasal, Pablo E.
dc.rights.holderRe Louhau, María F.
dc.rights.holderBottai, Hebe
dc.rights.holderKomloš, Kristina Fujs
dc.rights.holderPoljak, Mario
dc.rights.holderGiri, Adriana Angélica
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGammapapillomavirus
dc.subjectMucosal epithelia
dc.subjectGamma-PV/CUT PCR assays
dc.subjectAnal canal
dc.subjectPrevalence
dc.subjectPersistence
dc.titleAssessing Gammapapillomavirus infections of mucosal epithelia with two broad-spectrum PCR protocols
dc.typearticulo
dc.type.versionpublishedVersion

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