The role of cell-envelope synthesis for envelope growth and cytoplasmic density in Bacillus subtilis

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dc.citation.titlePNAS Nexus
dc.contributor.orcidhttp://orcid.org/0000-0001-8034-9058
dc.contributor.orcidhttp://orcid.org/0000-0002-2813-0259
dc.contributor.orcidhttp://orcid.org/0000-0002-7216-9804
dc.contributor.orcidhttp://orcid.org/0000-0002-1309-753X
dc.contributor.orcidhttp://orcid.org/0000-0003-0141-3555
dc.contributor.orcidhttp://orcid.org/0000-0002-0877-1294
dc.creatorKitahara, Yuki
dc.creatorOldewurtel, Enno R.
dc.creatorWilson, Sean
dc.creatorSun, Yingjie
dc.creatorAltabe, Silvia Graciela
dc.creatorDe Mendoza, Diego
dc.creatorGarner, Ethan C.
dc.creatorVan Teeffelen, Sven
dc.date.accessioned2024-06-24T18:12:32Z
dc.date.available2024-06-24T18:12:32Z
dc.date.issued2022-07-26
dc.description.abstractAll cells must increase their volumes in response to biomass growth to maintain intracellular mass density within physiologically permissive bounds. Here, we investigate the regulation of volume growth in the Gram-positive bacterium Bacillus subtilis. To increase volume, bacteria enzymatically expand their cell envelopes and insert new envelope material. First, we demonstrate that cell-volume growth is determined indirectly, by expanding their envelopes in proportion to mass growth, similarly to the Gram-negative Escherichia coli, despite their fundamentally different envelope structures. Next, we studied, which pathways might be responsible for robust surface-to-mass coupling: We found that both peptidoglycan synthesis and membrane synthesis are required for proper surface-tomass coupling. However, surprisingly, neither pathway is solely rate-limiting, contrary to wide-spread belief, since envelope growth continues at a reduced rate upon complete inhibition of either process. To arrest cell-envelope growth completely, the simultaneous inhibition of both envelope-synthesis processes is required. Thus, we suggest that multiple envelope-synthesis pathways collectively confer an important aspect of volume regulation, the coordination between surface growth, and biomass growth.
dc.description.filFil: Kitahara, Yuki. Université de Montréal. Faculté de Médecine. Département de Microbiologie, Infectiologie, et Immunologie; Canada.
dc.description.filFil: Kitahara, Yuki. Université de Paris; France.
dc.description.filFil: Kitahara, Yuki. Institut Pasteur. Microbial Morphogenesis and Growth Lab; France.
dc.description.filFil: Oldewurtel, Enno R. Institut Pasteur. Microbial Morphogenesis and Growth Lab; France.
dc.description.filFil: Wilson, Sean. Harvard University. Department of Molecular and Cellular Biology; USA.
dc.description.filFil: Wilson, Sean. Harvard University. Center for Systems Biology; USA.
dc.description.filFil: Sun, Yingjie. Harvard University. Department of Molecular and Cellular Biology; USA.
dc.description.filFil: Sun, Yingjie. Harvard University. Center for Systems Biology; USA.
dc.description.filFil: Altabe, Silvia Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.filFil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina.
dc.description.filFil: Garner, Ethan C. Harvard University. Department of Molecular and Cellular Biology; USA.
dc.description.filFil: Garner, Ethan C. Harvard University. Center for Systems Biology; USA.
dc.description.filFil: Van Teeffelen, Sven. Université de Montréal. Faculté de Médecine. Département de Microbiologie, Infectiologie, et Immunologie; Canada.
dc.description.filFil: Kitahara, Yuki. Université de Montréal. Faculté de Médecine. Département de Microbiologie, Infectiologie, et Immunologie; Canada. Fil: Kitahara, Yuki. Université de Paris; France. Fil: Kitahara, Yuki. Institut Pasteur. Microbial Morphogenesis and Growth Lab; France. Fil: Oldewurtel, Enno R. Institut Pasteur. Microbial Morphogenesis and Growth Lab; France. Fil: Wilson, Sean. Harvard University. Department of Molecular and Cellular Biology; USA. Fil: Wilson, Sean. Harvard University. Center for Systems Biology; USA. Fil: Sun, Yingjie. Harvard University. Department of Molecular and Cellular Biology; USA. Fil: Sun, Yingjie. Harvard University. Center for Systems Biology; USA. Fil: Altabe, Silvia Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina. Fil: De Mendoza, Diego. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario. (IBR-CONICET); Argentina. Fil: Garner, Ethan C. Harvard University. Department of Molecular and Cellular Biology; USA. Fil: Garner, Ethan C. Harvard University. Center for Systems Biology; USA. Fil: Van Teeffelen, Sven. Université de Montréal. Faculté de Médecine. Département de Microbiologie, Infectiologie, et Immunologie; Canada. Fil: Van Teeffelen, Sven. Institut Pasteur. Microbial Morphogenesis and Growth Lab; France.
dc.description.sponsorshipHorizon 2020 Framework Programme: 679980
dc.description.sponsorshipNational Institutes of Health: DP2AI117923-01
dc.format.extent1-16
dc.identifier.issn2752-6542
dc.identifier.urihttps://hdl.handle.net/2133/27337
dc.language.isoen
dc.publisherOxford University Press
dc.relation.publisherversionhttps://doi.org/10.1093/pnasnexus/pgac134
dc.relation.publisherversionhttps://academic.oup.com/pnasnexus/article/1/4/pgac134/6650235?login=true
dc.rightsopenAccess
dc.rights.holderKitahara, Yuki
dc.rights.holderOldewurtel, Enno R.
dc.rights.holderWilson, Sean
dc.rights.holderSun, Yingjie
dc.rights.holderAltabe, Silvia Graciela
dc.rights.holderDe Mendoza, Diego
dc.rights.holderGarner, Ethan C.
dc.rights.holderVan Teeffelen, Sven
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCell envelope
dc.subjectGrowth
dc.subjectBacillus subtilis
dc.titleThe role of cell-envelope synthesis for envelope growth and cytoplasmic density in Bacillus subtilis
dc.typearticulo
dc.type.versionpublishedVersion

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