Microevolution in the major outer membrane protein OmpA of Acinetobacter baumannii

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dc.citation.titleMicrobial Genomics
dc.citation.volume6(6)
dc.creatorViale, Alejandro M.
dc.creatorEvans, Benjamin A.
dc.date.accessioned2021-04-20T00:14:54Z
dc.date.available2021-04-20T00:14:54Z
dc.date.issued2020-06-04
dc.descriptionAcinetobacter baumannii is nowadays a relevant nosocomial pathogen characterized by multidrug resistance (MDR) and concomitant difficulties to treat infections. OmpA is the most abundant A. baumannii outer membrane (OM) protein, and is involved in virulence, host-cell recognition, biofilm formation, regulation of OM stability, permeability and antibiotic resistance. OmpA members are twoā€domain proteins with an Nā€terminal eightā€stranded Ī²ā€barrel domain with four external loops (ELs) interact ing with the environment, and a Cā€terminal periplasmic domain binding nonā€covalently to the peptidoglycan. Here, we combined data from genome sequencing, phylogenetic and multilocus sequence analyses from 975 strains/isolates of the Acinetobacter calcoaceticus/Acinetobacter baumannii complex (ACB), 946 from A. baumannii, to explore ompA microevolutionary divergence. Five major ompA variant groups were identified (V1 to V5) in A. baumannii, encompassing 52 different alleles coding for 23 different proteins. Polymorphisms were concentrated in five regions corresponding to the four ELs and the Cā€terminal end, and provided evidence for intraā€genic recombination. ompA variants were not randomly distributed across the A. baumannii phylogeny, with the most frequent V1(lct)a1 allele found in most clonal complex 2 (CC2) strains and the second most frequent V2(lct)a1 allele in the majority of CC1 strains. Evidence was found for assortative exchanges of ompA alleles not only between separate A. baumannii lineages, but also different ACB species. The overall results have implications for A. baumannii evolution, epidemiology, virulence and vaccine design.es
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquƭmicas y FarmacƩuticas. Instituto de Biologƭa Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquƭmicas y FarmacƩuticas. Departamento de Microbiologƭa; Argentina.
dc.description.filFil: Evans, Benjamin A. University of East Anglia. Norwich Medical School; United Kingdom.
dc.description.sponsorshipAgencia Nacional de PromociĆ³n CientĆ­fica y TecnolĆ³gica (ANPCyT): PICTā€2015ā€1072es
dc.description.sponsorshipConsejo Nacional de Investigaciones CientĆ­ficas y TĆ©cnicas (CONICET)es
dc.description.sponsorshipMinisterio de Salud de Santa Fees
dc.formatapplication/pdf
dc.format.extent1-14
dc.identifier.issn2057-5858
dc.identifier.urihttp://hdl.handle.net/2133/20517
dc.language.isoenges
dc.publisherMicrobiology Societyes
dc.relation.publisherversionhttps://doi.org/10.1099/mgen.0.000381es
dc.relation.publisherversionhttps://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000381es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderViale, Alejandro M.es
dc.rights.holderEvans, Benjamin A.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAcinetobacter baumanniies
dc.subjectProtein Evolutiones
dc.subjectOmpAes
dc.subjectRecombinationes
dc.titleMicroevolution in the major outer membrane protein OmpA of Acinetobacter baumanniies
dc.typearticle
dc.typeartĆ­culo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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