Transfection of Capsaspora owczarzaki, a close unicellular relative of animals

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dc.citation.titleDevelopment
dc.citation.volume145
dc.contributor.orcidhttps://orcid.org/0000-0003-0897-0186
dc.contributor.orcidhttps://orcid.org/0000-0001-6547-5304
dc.creatorParra Acero, Helena
dc.creatorRos Rocher, Núria
dc.creatorPerez Posada, Alberto
dc.creatorKozyczkowska, Aleksandra
dc.creatorSánchez Pons, Núria
dc.creatorNakata, Azusa
dc.creatorSuga, Hiroshi
dc.creatorNajle, Sebastián R.
dc.creatorRuiz Trillo, Iñaki
dc.date.accessioned2024-12-09T14:50:38Z
dc.date.available2024-12-09T14:50:38Z
dc.date.issued2018
dc.description.abstractHow animals emerged from their unicellular ancestor remains a major evolutionary question. New genome data from the closest unicellular relatives of animals have provided important insights into the evolution of animal multicellularity. We know that the unicellular ancestor of animals had an unexpectedly complex genetic repertoire, including many genes that are key to animal development and multicellularity. Thus, assessing the function of these genes among unicellular relatives of animals is key to understanding how they were co-opted at the onset of the Metazoa. However, such analyses have been hampered by the lack of genetic tools. Progress has been made in choanoflagellates and teretosporeans, two of the three lineages closely related to animals, whereas no tools are yet available for functional analysis in the third lineage: the filastereans. Importantly, filastereans have a striking repertoire of genes involved in transcriptional regulation and other developmental processes. Here, we describe a reliable transfection method for the filasterean Capsaspora owczarzaki. We also provide a set of constructs for visualising subcellular structures in live cells. These tools convert Capsaspora into a unique experimentally tractable organism to use to investigate the origin and evolution of animal multicellularity.
dc.description.filFil: Parra Acero, Helena. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Ros Rocher, Núria. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Perez Posada, Alberto. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Kozyczkowska, Aleksandra. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Sánchez Pons, Núria. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Nakata, Azusa. Prefectural University of Hiroshima. Faculty of Life and Environmental Sciences; Japan.
dc.description.filFil: Suga, Hiroshi. Prefectural University of Hiroshima. Faculty of Life and Environmental Sciences; Japan.
dc.description.filFil: Najle, Sebastián R. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Najle, Sebastián R. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ruiz Trillo, Iñaki. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Ruiz Trillo, Iñaki. Universitat de ́ Barcelona. Departament de Genè tica, Microbiologia i Estadıstica; Spain.
dc.description.filFil: Ruiz Trillo, Iñaki. Institució Catalana de Recerca i Estudis Avançats; Spain.
dc.description.sponsorshipEuropean Research Council: ERC-2007-StG-206883,ERC-2012-Co-616960
dc.description.sponsorshipMinisterio de Economía y Competitividad: BFU2014-57779-P
dc.description.sponsorshipGeneralitat de Catalunya Project: 2014 SGR 619
dc.description.sponsorshipMinisterio de Educación, Cultura y Deporte: FPU13/01840
dc.description.sponsorshipJapan Society for the Promotion of Science: KAKENHI 16K07468
dc.description.versionpeerreviewed
dc.format.extent1-8
dc.identifier.citationParra Acero, Helena; Ros Rocher, Núria; Perez Posada, Alberto; Kożyczkowska, Aleksandra; Sánchez Pons, Núria; et al.; Transfection of Capsaspora owczarzaki, a close unicellular relative of animals; Company of Biologists; Development; 145; 10; 5-2018; 1-27
dc.identifier.issn0950-1991
dc.identifier.urihttps://hdl.handle.net/2133/28352
dc.language.isoen
dc.publisherThe Company of Biologists
dc.relation.publisherversionhttps://doi.org/10.1242/dev.162107
dc.relation.publisherversionhttps://journals.biologists.com/dev/article/145/10/dev162107/48545/Transfection-of-Capsaspora-owczarzaki-a-close
dc.rightsopenAccess
dc.rights.holderThe Company of Biologists Ltd
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTransfection
dc.subjectCapsaspora owczarzaki
dc.subjectHolozoa
dc.subjectMulticellularity
dc.subjectOrigin of metazoa
dc.titleTransfection of Capsaspora owczarzaki, a close unicellular relative of animals
dc.typearticulo
dc.type.versionpublishedVersion

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