Staring at the cold sun: blue light regulation is distributed within the genus acinetobacter

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dc.citation.titlePLoS ONE
dc.citation.volume8
dc.creatorGolic, Adrián Ezequiel
dc.creatorVaneechoutte, Mario
dc.creatorNemec, Alexandr
dc.creatorViale, Alejandro M.
dc.creatorActis, Luis A.
dc.creatorMussi, María Alejandra
dc.date.accessioned2024-08-06T14:49:13Z
dc.date.available2024-08-06T14:49:13Z
dc.date.issued2013-01-24
dc.description.abstractWe previously showed that the opportunistic nosocomial pathogen Acinetobacter baumannii is able to sense and respond to light via BlsA, a BLUF (Blue-Light-sensing Using FAD)-domain photoreceptor protein. Here, we extend our previous studies showing that light regulation is not restricted to A. baumannii, but rather widespread within the genus Acinetobacter. First, we found that blue light modulates motility and biofilm formation in many species of the genus, including members of the Acinetobacter calcoaceticus-A. baumannii complex. In many of these species blue light acts as a key factor guiding the decision between motility or sessility at 24°C, whereas in A. baumannii, light inhibits both motility and biofilm formation. We also show that light regulation of motility occurred not only at 24°C but also at 37°C in non-A. baumannii species, contrasting the situation of A. baumannii which only shows photoregulation at 24°C. Second, we show that Acinetobacter baylyi (strain ADP1) BLUF-photoreceptors can functionally replace in vivo the A. baumannii 17978 BlsA protein and that the pathways leading to biofilm formation are inversely regulated at 24°C between these two microorganisms. Finally, we found the presence of predicted genes coding BLUF-containing proteins in all Acinetobacter sequenced genomes, even though the copy number is variable among them. Phylogenetic analysis suggests a common origin for all BLUF domains present in members of this genus, and could distinguish well-differentiated clusters that group together BLUF homologs from different species, a situation particularly clear for members of the ACB complex. Despite a role played by these BLUF domain-containing proteins in the photoregulation observed in the members of the genus Acinetobacter is a likely scenario given our findings in A. baumannii and A. baylyi, further research will contribute to confirm this possibility.
dc.description.filFil: Golic, Adrián Ezequiel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Vaneechoutte, Mario. Ghent University. Ghent University Hospital. Laboratory for Bacteriology Research; Belgium.
dc.description.filFil: Nemec, Alexandr. National Institute of Public Health. Laboratory of Bacterial Genetics; Czech Republic.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Actis, Luis A. Miami University. Department of Microbiology; United States.
dc.description.filFil: Mussi, María Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 326 2011–2013, PIP 114–200801–00469
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 25972
dc.description.sponsorshipSecretaría de Estado de Ciencia, Tecnología e Innovación de la Provincia de Santa Fe: grant 2012
dc.description.versionpeerreviewed
dc.format.extent1-14
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2133/27516
dc.language.isoen
dc.publisherPublic Library of Science (PLOS)
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0055059
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055059
dc.rightsopenAccess
dc.rights.holderGolic, Adrián Ezequiel
dc.rights.holderVaneechoutte, Mario
dc.rights.holderNemec, Alexandr
dc.rights.holderViale, Alejandro M.
dc.rights.holderActis, Luis A.
dc.rights.holderMussi, María Alejandra
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAcinetobacter baumannii
dc.subjectAcinetobacter calcoaceticus
dc.subjectAcinetobacter baylyi
dc.subjectBlsA protein
dc.subjectBlue light
dc.subjectBLUF domain
dc.subjectLight regulation
dc.subjectPhotoregulation
dc.subjectBiofilms
dc.subjectMotility
dc.titleStaring at the cold sun: blue light regulation is distributed within the genus acinetobacter
dc.typearticulo
dc.type.versionpublishedVersion

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