Activation of spleen tyrosine kinase (Syk) at fertilization in Rhinella arenarum eggs

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dc.citation.titleThe International Journal of Developmental Biology
dc.citation.volume58
dc.creatorMouguelar, Valeria Soraya
dc.creatorCoux, Gabriela
dc.date.accessioned2024-11-19T14:40:23Z
dc.date.available2024-11-19T14:40:23Z
dc.date.issued2015-07-02
dc.description.abstractRecently, we have provided evidence for the involvement of a cytosolic tyrosine–phosphorylatable 70 kDa oocyte protein in Rhinella arenarum (Anura: Bufonidae) fertilization. The aim of the present work was to characterize its phosphorylation, determine the identity of this protein and establish its biological role during the fertilization process. Tyrosine phosphorylation of the 70 kDa protein was not observed in eggs activated with the calcium ionophore A23187. Pretreatment of oocytes with the tyrosine kinase inhibitor genistein effectively blocked the fertilization-dependent phosphorylation of the 70 kDa protein. In order to identify this protein, we examined the presence in amphibian oocytes of non-receptor 70 kDa tyrosine kinase members of the Syk/Zap70 and Tec families by RT-PCR using degenerate primers. We found that R. arenarum oocytes contain the transcripts coding for Syk and Tec kinases. Western blot analysis confirmed the presence of Syk protein in unfertilized oocytes and eggs. Studies using phospho-Syk specific antibodies showed that fertilization rapidly (less than 10 minutes) induces phosphorylation on Syk tyrosine residues (352 and 525/526) that are necessary for the activation of the enzyme. Finally, specific inhibition of Syk with the R406 compound provoked a diminished fertilization score, thereby confirming a functional role of the Syk protein during R. arenarum fertilization. To our knowledge this is the first time that Syk is described as a player in the signaling cascade activated after fertilization.
dc.description.filFil: Mouguelar, Valeria Soraya. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Coux, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.versionpeerreviewed
dc.format.extent1-9
dc.identifier.citationMouguelar, V. S. and Coux, G. (2015). Activation of spleen tyrosine kinase (Syk) at fertilization in Rhinella arenarum eggs. The International Journal of Developmental Biology, 58, 811-818. https://doi.org/10.1387/ijdb.140302gc
dc.identifier.e-issn1696-3547
dc.identifier.issn0214-6282
dc.identifier.urihttps://hdl.handle.net/2133/28161
dc.language.isoen
dc.publisherUniversity of the Basque Country Press (UBC Press)
dc.relation.publisherversionhttps://doi.org/10.1387/ijdb.140302gc
dc.relation.publisherversionhttps://ijdb.ehu.eus/article/140302gc
dc.rightsopenAccess
dc.rights.holderMouguelar, Valeria Soraya
dc.rights.holderCoux, Gabriela
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTyrosine phosphorylation
dc.subjectAmphibian
dc.subjectSignaling
dc.subjectFertilization
dc.subjectSyk Kinase
dc.subjectRhinella arenarum
dc.subjectSpleen tyrosine kinase (SYK)
dc.subjectPhosphorylation
dc.titleActivation of spleen tyrosine kinase (Syk) at fertilization in Rhinella arenarum eggs
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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