The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis

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dc.citation.titlePLOS ONEes
dc.creatorHernández-Pando, Rogelio
dc.creatorBarrios-Payán, Jorge
dc.creatorMata-Espinosa, Dulce
dc.creatorMarquina-Castillo, Brenda
dc.creatorHernández-Ramírez, Diego
dc.creatorBottasso, Oscar
dc.creatorBini, Estela Isabel
dc.date.accessioned2015-08-03T13:35:24Z
dc.date.available2015-08-03T13:35:24Z
dc.date.issued2015-07-22
dc.descriptionBackground The high mobility group box 1 (HMGB1) is the prototype of alarmin protein released by stressed or dying cells. The redox state of this protein confers different functions in the regulation of inflammation and immune response. Aim Determine the kinetics, cellular sources and function of HMGB1 in experimental tuberculosis. Methods BALB/c mice were infected with Mycobacterium tuberculosis strain H37Rv. At different time points, HMGB1 was quantified in bronchial lavage fluid (BALF) and in lungs was determined its cellular sources by immunohistochemistry. HMGB1 was blocked with specific antibodies or recombinant HMGB1 was administered during early or late infection. Bacilli burdens, inflammation and cytokines expression were determined. Results The maximal concentration of HMGB1 in BALF was at day one of infection. Bronchial epithelium and macrophages were the most important sources. At day 7 to 21 the oxidized HMGB1 was predominant, while during late infection only the reduced form was seen. Blocking HMGB1 during early infection produced significant decrease of bacilli burdens and high production of pro-inflammatory cytokines, while the opposite was seen when HMGB1 was administered. Blocking HMGB1 activity or administrated it in high amounts during late infection worsening the disease. Conclusions HMGB1 is liberated during experimental tuberculosis and promotes or suppress the immune response and inflammation depending on the redox state.es
dc.description.filFil: Bottasso, Oscar. Institute of Experimental and Clinic Immunology, Rosario, IDICER, CONICET, School of Medical Sciences. UNR. Rosario; Argentinaes
dc.description.filFil: Bini, Estela Isabel. Institute of Experimental and Clinic Immunology, Rosario, IDICER, CONICET, School of Medical Sciences. UNR. Rosario; Argentinaes
dc.description.sponsorshipEstela Isabel Bini was awarded with a travelling fellowship from DMM Company of Biologists, England. This work was supported by the Bilateral International Project Argentina/México, CONICET/CONACYT, number 190527.es
dc.formatapplication/pdf
dc.format.extent1-14es
dc.identifier.issn1932-6203es
dc.identifier.urihttp://hdl.handle.net/2133/4868
dc.language.isoenges
dc.publisherPLOS (Public Library of Science)es
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0133200es
dc.rightsopenAccesses
dc.rightshttp://creativecommons.org/licenses/by/4.0/
dc.rights.holder© 2015 Hernández-Pando et al.es
dc.rights.textThis is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.es
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAntibodieses
dc.subjectInflammationes
dc.subjectCytokineses
dc.subjectMacrophageses
dc.subjectMycobacterium tuberculosises
dc.subjectImmunostaininges
dc.subjectEnzyme-linked immunoassayses
dc.titleThe Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosises
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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FCM - Instituto de Inmunología - Artículos de Investigación
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