Dynamic state of plasmid genomic architectures resulting from XerC/D-mediated site-specific recombination in Acinetobacter baumannii Rep_3 superfamily resistance plasmids carrying blaOXA-58- and TnaphA6-resistance modules

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dc.citation.titleFrontiers in Microbiology
dc.citation.volume14
dc.creatorGiacone, Lucía
dc.creatorCameranesi, María Marcela
dc.creatorSánchez, Rocio Inés
dc.creatorLimansky, Adriana S.
dc.creatorMorán Barrio, Jorgelina
dc.creatorViale, Alejandro M.
dc.date.accessioned2024-10-24T18:49:38Z
dc.date.available2024-10-24T18:49:38Z
dc.date.issued2023-02-09
dc.description.abstractThe acquisition of blaOXA genes encoding different carbapenem-hydrolyzing class-D β-lactamases (CHDL) represents a main determinant of carbapenem resistance in the nosocomial pathogen Acinetobacter baumannii. The blaOXA-58 gene, in particular, is generally embedded in similar resistance modules (RM) carried by plasmids unique to the Acinetobacter genus lacking self-transferability. The ample variations in the immediate genomic contexts in which blaOXA-58-containing RMs are inserted among these plasmids, and the almost invariable presence at their borders of nonidentical 28-bp sequences potentially recognized by the host XerC and XerD tyrosine recombinases (pXerC/D-like sites), suggested an involvement of these sites in the lateral mobilization of the gene structures they encircle. However, whether and how these pXerC/D sites participate in this process is only beginning to be understood. Here, we used a series of experimental approaches to analyze the contribution of pXerC/D-mediated site-specific recombination to the generation of structural diversity between resistance plasmids carrying pXerC/D-bounded blaOXA-58- and TnaphA6-containing RM harbored by two phylogenetically- and epidemiologicallyclosely related A. baumannii strains of our collection, Ab242 and Ab825, during adaptation to the hospital environment. Our analysis disclosed the existence of different bona fide pairs of recombinationally-active pXerC/D sites in these plasmids, some mediating reversible intramolecular inversions and others reversible plasmid fusions/resolutions. All of the identified recombinationally-active pairs shared identical GGTGTA sequences at the cr spacer separating the XerC- and XerD-binding regions. The fusion of two Ab825 plasmids mediated by a pair of recombinationally-active pXerC/D sites displaying sequence differences at the cr spacer could be inferred on the basis of sequence comparison analysis, but no evidence of reversibility could be obtained in this case. The reversible plasmid genome rearrangements mediated by recombinationally-active pairs of pXerC/D sites reported here probably represents an ancient mechanism of generating structural diversity in the Acinetobacter plasmid pool. This recursive process could facilitate a rapid adaptation of an eventual bacterial host to changing environments, and has certainly contributed to the evolution of Acinetobacter plasmids and the capture and dissemination of blaOXA-58 genes among Acinetobacter and non-Acinetobacter populations co-residing in the hospital niche.
dc.description.filFil: Giacone, Lucía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.filFil: Cameranesi, María Marcela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.filFil: Sánchez, Rocio Inés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.filFil: Limansky, Adriana S. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.filFil: Morán Barrio, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología. Instituto de Biología Molecular y Celular de Rosario (IBR); Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT-2017-3536, PICT-2019-00074
dc.format.extent1-13
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/2133/27980
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2023.1057608
dc.relation.publisherversionhttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1057608/full
dc.rightsopenAccess
dc.rights.holderGiacone, Lucía
dc.rights.holderCameranesi, María Marcela
dc.rights.holderSánchez, Rocio Inés
dc.rights.holderLimansky, Adriana S.
dc.rights.holderMorán Barrio, Jorgelina
dc.rights.holderViale, Alejandro M.
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAcinetobacter baumannii
dc.subjectCarbapenem resistance
dc.subjectResistance plasmids
dc.subjectOXA-58 carbapenemase
dc.subjectXerC/D site-specific recombination
dc.subjectMultiple pXerC/D site
dc.subjectPlasmid shuffling
dc.titleDynamic state of plasmid genomic architectures resulting from XerC/D-mediated site-specific recombination in Acinetobacter baumannii Rep_3 superfamily resistance plasmids carrying blaOXA-58- and TnaphA6-resistance modules
dc.typearticulo
dc.type.versionpublishedVersion

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