Low cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA strain

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dc.citation.titleApplied Microbiology and Biotechnologyes
dc.creatorCerminati, Sebastián
dc.creatorLeroux, Mélanie
dc.creatorAnselmi, Pablo Ariel
dc.creatorPeirú, Salvador
dc.creatorAlonso, Juan C.
dc.creatorPriem, Bernard
dc.creatorMenzella, Hugo G.
dc.date.accessioned2022-03-29T16:13:31Z
dc.date.available2022-03-29T16:13:31Z
dc.date.issued2021-04-05
dc.descriptionHyaluronic acid (HA) is a high value glycosaminoglycan mostly used in health and cosmetic applications. Commercial HA is produced from animal tissues or in toxigenic bacteria of the genus Streptococcus grown in complex media, which are expensive and raise environmental concerns due to the disposal of large amounts of broth with high organic loads. Other microorganisms were proposed as hosts for the heterologous production of HA, but the methods are still costly. The extraordinary capacity of this biopolymer to bind and retain water attracts interest for large-scale applications where biodegradable materials are needed, but its high cost and safety concerns are barriers for its adoption. Bacillus subtilis 3NA strain is prototrophic, amenable for genetic manipulation, GRAS, and can rapidly reach high cell densities in salt-based media. These phenotypic traits were exploited to create a platform for biomolecule production using HA as a proof of concept. First, the 3NA strain was engineered to produce HA; second, a chemically defined medium was formulated using commodity-priced inorganic salts combined at the stoichiometric ratios needed to build the necessary quantities of biomass and HA; and third, a scalable fermentation process, where HA can be produced at the maximum volumetric productivity (VP), was designed. A comparative economic analysis against other methods indicates that the new process may increase the operating profit of a manufacturing plant by more than 100%. The host, the culture medium, and the rationale employed to develop the fermentation process described here, introduce an IP free platform that could be adaptable for production of other biomolecules.es
dc.description.filFil: Cerminati, Sebastián. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina.es
dc.description.filFil: Leroux, Mélanie. Université Grenoble Alpes. Centre National de la Recherche Scientifique. Centre de Recherches sur les Macromolécules Végétales; France.es
dc.description.filFil: Anselmi, Pablo Ariel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina.es
dc.description.filFil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina.es
dc.description.filFil: Alonso, Juan C. Centro Nacional de Biotecnología. Department of Microbial Biotechnology; Spain.es
dc.description.filFil: Priem, Bernard. Université Grenoble Alpes. Centre National de la Recherche Scientifique. Centre de Recherches sur les Macromolécules Végétales; France.es
dc.description.filFil: Menzella, Hugo G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos (IPROBYQ-CONICET); Argentina.es
dc.formatapplication/pdf
dc.format.extent3075–3086es
dc.identifier.issn1432-0614es
dc.identifier.urihttp://hdl.handle.net/2133/23283
dc.language.isoenges
dc.publisherSpringeres
dc.relation.publisherversionhttps://doi.org/10.1007/s00253-021-11246-6
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00253-021-11246-6#Ack1
dc.rightsopenAccesses
dc.rights.holderCerminati, Sebastiánes
dc.rights.holderLeroux, Mélaniees
dc.rights.holderAnselmi, Pablo Arieles
dc.rights.holderPeirú, Salvadores
dc.rights.holderAlonso, Juan C.es
dc.rights.holderPriem, Bernardes
dc.rights.holderMenzella, Hugo G.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectHyaluronic acides
dc.subjectGreen chemistryes
dc.subjectBacillus subtilises
dc.subjectFermentationes
dc.titleLow cost and sustainable hyaluronic acid production in a manufacturing platform based on Bacillus subtilis 3NA straines
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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