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Study of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopy

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dc.citation.titleProceedings of SPIEes
dc.citation.volumePROCEEDINGS VOLUME 11251 SPIE BIOS 1-6 February 2020es
dc.contributor.organizerSociety of Photo-optical Instrumentation Engineers (SPIE)es
dc.creatorToderi, Martín A.
dc.creatorGalizzi, Gustavo E.
dc.creatorRiquelme, Bibiana Doris
dc.creatorDumas, Dominique
dc.date.accessioned2020-03-02T23:08:34Z
dc.date.available2020-03-02T23:08:34Z
dc.date.issued2020-02-20
dc.description.abstractIn this work, we studied the nature of the molecular bonds involved in the red blood cell aggregation process by the coherent anti-Stokes Raman spectroscopy technique. Images were acquired with a commercial Leica TCS SP8 CARS confocal microscope (Leica Microsystems GmbH, Wetzlar, Germany) temporally and spatially overlapping the pulses of two sources in the focal plane of the microscope. A pump wavelength of 810 nm to 817 nm was used for the CARS mode simultaneously with the Stokes beam at 1064 nm to excite the vibratory resonance of the symmetric hydrocarbon bonds in the lipids and that of the bonds in amino acids of the proteins. The Raman shift was also observed at the 1200 cm−1 range to study possible variations in the sialic acid on the cell membrane produced by concentrations of dextran 500 in the suspension medium. Curves of lifetime emission distribution were obtained for untreated erythrocytes and treated erythrocytes with dextran 500, particularly at a pump wavelength of 904 nm.es
dc.description.filFil: Toderi, Martin. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.filFil: Toderia, Martin A. Instituto de Física Rosario (CONICET-UNR); Argentina.es
dc.description.filFil: Galizzi, Gustavo E. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.es
dc.description.filFil: Galizzi, Gustavo E. Instituto de Física Rosario (CONICET-UNR); Argentina.es
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.filFil: Dumas, Dominique. Université de Lorraine. Ingénierie Moléculaire et Physiopathologie Articulaire; Francia.es
dc.description.filFil: Dumas, Dominique. Institut National de la Santé et de la Recherche Médicale; Franciaes
dc.description.filFil: Dumas, Dominique. Centre national de la Recherche Scientifique (CNRS); Francia.es
dc.description.sponsorshipCPER IT2MP IMTI A1 25 Francees
dc.description.sponsorshipFEDER Union Europ´eenne – Region Grand ESTes
dc.formatapplication/pdf
dc.format.extent1125124es
dc.identifier.issn1996-756Xes
dc.identifier.urihttp://hdl.handle.net/2133/17698
dc.language.isoenges
dc.publisherNatan T. Shaked y Oliver Haydenes
dc.relation.publisherversionhttps://www.spiedigitallibrary.org/conference-proceedings-of-spie/11251/1125124/Study-of-the-red-blood-cell-aggregation-by-coherent-anti/10.1117/12.2543073.shortes
dc.relation.publisherversionhttp://dx.doi.org/10.1117/12.2543073es
dc.rightsembargoedAccesses
dc.rights.holderToderi, Martín A.es
dc.rights.holderGalizzi, Gustavo E.es
dc.rights.holderRiquelme, Bibiana Dorises
dc.rights.holderDumas, Dominiquees
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticases
dc.rights.holderUniv. de Lorrainees
dc.rights.holderCNRS (France)es
dc.rights.holderInstitut National de la Santé et de la Recherche Médicale (France)es
dc.rights.holderSociety of Photo-Optical Instrumentation Engineers (SPIE)es
dc.rights.textAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/deed.es*
dc.subjectErythrocyte Aggregationes
dc.subjectCoherent anti-Stokes Raman Spectroscopyes
dc.subjectDextranes
dc.titleStudy of the red blood cell aggregation by coherent anti-Stokes Raman spectroscopyes
dc.typeconferenceObject
dc.typedocumento de conferencia
dc.typepublishedVersion
dc.type.collectioncomunicaciones
dc.type.versionpublishedVersiones

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