The dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses

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dc.citation.titleJournal of Experimental Botany
dc.citation.volume66
dc.creatorPiazza, Ainelén
dc.creatorZimaro, Tamara
dc.creatorGaravaglia, Betiana Soledad
dc.creatorFicarra, Florencia Andrea
dc.creatorThomas, Ludivine
dc.creatorMarondedze, Claudius
dc.creatorFeil, Regina
dc.creatorLunn, John E.
dc.creatorGehring, Chris
dc.creatorOttado, Jorgelina
dc.creatorGottig, Natalia
dc.date.accessioned2024-05-21T20:02:13Z
dc.date.available2024-05-21T20:02:13Z
dc.date.issued2015-03-15
dc.description.abstractXanthomonas citri subsp. citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp. The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis. Expression of genes encoding trehalose-6-phosphate synthase (otsA) and trehalose phosphatase (otsB) was highly induced during canker development, suggesting that the two-step pathway of trehalose biosynthesis via trehalose-6-phosphate has a function in pathogenesis. This pathway was eliminated from the bacterium by deletion of the otsA gene. The resulting XccΔotsA mutant produced less trehalose than the wild-type strain, was less resistant to salt and oxidative stresses, and was less able to colonize plant tissues. Gene expression and proteomic analyses of infected leaves showed that infection with XccΔotsA triggered only weak defence responses in the plant compared with infection with Xcc, and had less impact on the host plant’s metabolism than the wild-type strain. These results suggested that trehalose of bacterial origin, synthesized via the otsA–otsB pathway, in Xcc, plays a role in modifying the host plant’s metabolism to its own advantage but is also perceived by the plant as a sign of pathogen attack. Thus, trehalose biosynthesis has both positive and negative consequences for Xcc. On the one hand, it enables this bacterial pathogen to survive in the inhospitable environment of the leaf surface before infection and exploit the host plant’s resources after infection, but on the other hand, it is a tell-tale sign of the pathogen’s presence that triggers the plant to defend itself against infection.
dc.description.filFil: Piazza, Ainelén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Zimaro, Tamara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Garavaglia, Betiana Soledad. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ficarra, Florencia Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gottig, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Thomas, Ludivine. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.filFil: Marondedze, Claudius. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.filFil: Gehring, Chris. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.filFil: Feil, Regina. Wissenschaftspark Golm. Max Planck Institute of Molecular Plant Physiology; Germany.
dc.description.filFil: Lunn, John E. Wissenschaftspark Golm. Max Planck Institute of Molecular Plant Physiology; Germany.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT2010-1507, PICT2010- 0300
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP2010-2012
dc.description.sponsorshipMax Planck Society
dc.description.versionpeerreviewed
dc.format.extent1-17
dc.identifier.e-issn1460-2431
dc.identifier.issn0022-0957
dc.identifier.urihttps://hdl.handle.net/2133/27080
dc.language.isoen
dc.publisherOxford University
dc.relation.publisherversionhttps://doi.org/10.1093/jxb/erv095
dc.relation.publisherversionhttps://academic.oup.com/jxb/article/66/9/2795/679809?login=true
dc.rightsopenAccess
dc.rights.holderPiazza, Ainelén
dc.rights.holderZimaro, Tamara
dc.rights.holderGaravaglia, Betiana Soledad
dc.rights.holderFicarra, Florencia Andrea
dc.rights.holderThomas, Ludivine
dc.rights.holderMarondedze, Claudius
dc.rights.holderFeil, Regina
dc.rights.holderLunn, John E.
dc.rights.holderGehring, Chris
dc.rights.holderOttado, Jorgelina
dc.rights.holderGottig, Natalia
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCanker
dc.subjectCitrus
dc.subjectDefence
dc.subjectotsA
dc.subjectTrehalose
dc.subjectXanthomonas
dc.titleThe dual nature of trehalose in citrus canker disease: a virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses
dc.typearticulo
dc.type.versionpublishedVersion

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