Reactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea

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dc.citation.titleThe Plant Journal
dc.citation.volume92
dc.creatorRossi, Franco R.
dc.creatorKrapp, Adriana R.
dc.creatorBisaro, Fabiana
dc.creatorMaiale, Santiago J.
dc.creatorPieckenstain, Fernando L.
dc.creatorCarrillo, Néstor
dc.date.accessioned2024-09-30T14:56:51Z
dc.date.available2024-09-30T14:56:51Z
dc.date.issued2017-10
dc.description.abstractReactive oxygen species (ROS) play fundamental roles in plant responses to pathogen infection, including modulation of cell death processes and defense-related gene expression. Cell death triggered as part of the hypersensitive response enhances resistance to biotrophic pathogens, but favors the virulence of necrotrophs. Even though the involvement of ROS in the orchestration of defense responses is well established, the relative contribution of specific subcellular ROS sources to plant resistance against microorganisms with different pathogenesis strategies is not completely known. The aim of this work was to investigate the role of chloroplastic ROS in plant defense against a typical necrotrophic fungus, Botrytis cinerea. For this purpose, we used transgenic Nicotiana tabacum (tobacco) lines expressing a plastid-targeted cyanobacterial flavodoxin (pfld lines), which accumulate lower chloroplastic ROS in response to different stresses. Tissue damage and fungal growth were significantly reduced in infected leaves of pfld plants, as compared with infected wild-type (WT) counterparts. ROS build-up triggered by Botrytis infection and associated with chloroplasts was significantly decreased (70–80%) in pfld leaves relative to the wild type. Phytoalexin accumulation and expression of pathogenesis-related genes were induced to a lower degree in pfld plants than in WT siblings. The impact of fungal infection on photosynthetic activity was also lower in pfld leaves. The results indicate that chloroplast-generated ROS play a major role in lesion development during Botrytis infection. This work demonstrates that the modulation of chloroplastic ROS levels by the expression of a heterologous antioxidant protein can provide a significant degree of protection against a canonical necrotrophic fungus.
dc.description.filFil: Rossi, Franco R. Universidad Nacional de General San Martín. Instituto de Investigaciones Biotecnológicas. Instituto Tecnológico (IIB-INTECH-CONICET); Argentina.
dc.description.filFil: Krapp, Adriana R. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Bisaro, Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Maiale, Santiago J. Universidad Nacional de General San Martín. Instituto de Investigaciones Biotecnológicas. Instituto Tecnológico (IIB-INTECH-CONICET); Argentina.
dc.description.filFil: Pieckenstain, Fernando L. Universidad Nacional de General San Martín. Instituto de Investigaciones Biotecnológicas. Instituto Tecnológico (IIB-INTECH-CONICET); Argentina.
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipNational Agency for the Promotion of Science and Technology: PICT 2012-2851,PICT 2012-1716,PICT 2014-3286
dc.format.extent761-773
dc.identifier.issn1365-313X
dc.identifier.urihttps://hdl.handle.net/2133/27901
dc.language.isoen
dc.publisherSociety for Experimental Biology
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/tpj.13718
dc.relation.publisherversionhttps://doi.org/10.1111/tpj.13718
dc.rightsopenAccess
dc.rights.holderThe Plant Journal
dc.rights.holderJohn Wiley & Sons Ltd
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectChloroplastic ros
dc.subjectPlant–microbe interactions
dc.subjectNecrotrophs
dc.subjectBotrytis cinerea
dc.subjectFlavodoxin
dc.subjectNicotiana tabacum
dc.titleReactive oxygen species generated in chloroplasts contribute to tobacco leaf infection by the necrotrophic fungus Botrytis cinerea
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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