Designer TALEs enable discovery of cell death-inducer genes

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dc.citation.titlePlant Physiology
dc.citation.volume195
dc.contributor.orcidhttps://orcid.org/0000-0001-6121-7930
dc.contributor.orcidhttps://orcid.org/0009-0001-2033-3430
dc.contributor.orcidhttps://orcid.org/0009-0005-3778-9900
dc.contributor.orcidhttps://orcid.org/0009-0008-0589-9867
dc.contributor.orcidhttps://orcid.org/0000-0002-1625-422X
dc.contributor.orcidhttps://orcid.org/0000-0002-7862-9509
dc.contributor.orcidhttps://orcid.org/0000-0003-0437-589X
dc.contributor.orcidhttps://orcid.org/0000-0001-8399-0107
dc.contributor.orcidhttps://orcid.org/0009-0000-9611-0316
dc.contributor.orcidhttps://orcid.org/0000-0001-6600-8177
dc.contributor.orcidhttps://orcid.org/0000-0001-5883-7506
dc.contributor.orcidhttps://orcid.org/0000-0002-3612-7914
dc.creatorRoeschlin, Roxana Andrea
dc.creatorAzad, Sepideh M.
dc.creatorGrove, René P.
dc.creatorChuan, Ana
dc.creatorGarcía, Lucila
dc.creatorNiñoles, Regina
dc.creatorUviedo, Facundo
dc.creatorVillalobos, Liara
dc.creatorMassimino, Maria E.
dc.creatorMarano, María Rosa
dc.creatorBoch, Jens
dc.creatorGadea, José
dc.date.accessioned2025-03-07T16:25:24Z
dc.date.available2025-03-07T16:25:24Z
dc.date.issued2024-09-09
dc.description.abstractTranscription activator-like effectors (TALEs) in plant-pathogenic Xanthomonas bacteria activate expression of plant genes and support infection or cause a resistance response. PthA4AT is a TALE with a particularly short DNA-binding domain harboring only 7.5 repeats which triggers cell death in Nicotiana benthamiana; however, the genetic basis for this remains unknown. To identify possible target genes of PthA4AT that mediate cell death in N. benthamiana, we exploited the modularity of TALEs to stepwise enhance their specificity and reduce potential target sites. Substitutions of individual repeats suggested that PthA4AT-dependent cell death is sequence specific. Stepwise addition of repeats to the C-terminal or N-terminal end of the repeat region narrowed the sequence requirements in promoters of target genes. Transcriptome profiling and in silico target prediction allowed the isolation of two cell death inducer genes, which encode a patatin-like protein and a bifunctional monodehydroascorbate reductase/carbonic anhydrase protein. These two proteins are not linked to known TALE-dependent resistance genes. Our results show that the aberrant expression of different endogenous plant genes can cause a cell death reaction, which supports the hypothesis that TALE-dependent executor resistance genes can originate from various plant processes. Our strategy further demonstrates the use of TALEs to scan genomes for genes triggering cell death and other relevant phenotypes.
dc.description.filFil: Roeschlin, Roxana Andrea. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Azad, Sepideh M. Universidad Politécnica de Valencia-CSIC. Instituto de Biología Molecular y celular de Plantas (IBMCP); España.
dc.description.filFil: Grove, René P. Leibniz Universität Hannover. Institute of Plant Genetics; Germany.
dc.description.filFil: Chuan, Ana. Universidad Politécnica de Valencia-CSIC. Instituto de Biología Molecular y celular de Plantas (IBMCP); España.
dc.description.filFil: García, Lucila. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: García, Lucila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Niñoles, Regina. Universidad Politécnica de Valencia-CSIC. Instituto de Biología Molecular y celular de Plantas (IBMCP); España.
dc.description.filFil: Uviedo, Facundo. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Villalobos, Liara. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Massimino, Maria E. Universidad Politécnica de Valencia-CSIC. Instituto de Biología Molecular y celular de Plantas (IBMCP); España.
dc.description.filFil: Marano, María Rosa. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Marano, María Rosa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Virología; Argentina.
dc.description.filFil: Boch, Jens. Leibniz Universität Hannover. Institute of Plant Genetics; Germany.
dc.description.filFil: Gadea, José. Universidad Politécnica de Valencia-CSIC. Instituto de Biología Molecular y celular de Plantas (IBMCP); España.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica: PICT-2020-01624
dc.description.sponsorshipEuropean Cooperation in Science and Technology
dc.format.extent2985–2996
dc.identifier.citationRoxana A Roeschlin, Sepideh M Azad, René P Grove, Ana Chuan, Lucila García, Regina Niñoles, Facundo Uviedo, Liara Villalobos, Maria E Massimino, María R Marano, Jens Boch, José Gadea, Designer TALEs enable discovery of cell death-inducer genes, Plant Physiology, Volume 195, Issue 4, August 2024, Pages 2985–2996, https://doi.org/10.1093/plphys/kiae230
dc.identifier.issn0032-0889
dc.identifier.urihttps://hdl.handle.net/2133/29020
dc.language.isoen
dc.publisherOxford University Press
dc.relation.publisherversionhttps://academic.oup.com/plphys/article/195/4/2985/7667890?login=true
dc.relation.publisherversionhttps://doi.org/10.1093/plphys/kiae230
dc.rightsopenAccess
dc.rights.holderRoeschlin, Roxana Andrea
dc.rights.holderAzad, Sepideh M.
dc.rights.holderGrove, René P.
dc.rights.holderChuan, Ana
dc.rights.holderGarcía, Lucila
dc.rights.holderNiñoles, Regina
dc.rights.holderUviedo, Facundo
dc.rights.holderVillalobos, Liara
dc.rights.holderMassimino, Maria E.
dc.rights.holderMarano, María Rosa
dc.rights.holderBoch, Jens
dc.rights.holderGadea, José
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectTranscription activator-like effectors
dc.subjectCell death
dc.subjectXanthomonas
dc.subjectGenes, plant
dc.titleDesigner TALEs enable discovery of cell death-inducer genes
dc.typearticulo
dc.type.versionpublishedVersion

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(FBIOyF) Departamento de Microbiología - Artículos