Genetic tool development in marine protists: emerging model organisms for experimental cell biology

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dc.citation.titleNature Methods
dc.citation.volume17
dc.creatorNajle, Sebastián R.
dc.creatorFaktorová, Drahomíra
dc.creatorNisbet, R. Ellen R.
dc.creatorFernández Robledo, José A.
dc.creatorCasacuberta, Elena
dc.creatorSudek, Lisa
dc.creatorAllen, Andrew E.
dc.date.accessioned2025-02-13T17:51:03Z
dc.date.available2025-02-13T17:51:03Z
dc.date.issued2020-04-15
dc.description.abstractDiverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways.
dc.description.filFil: Faktorová, Drahomíra. University of South Bohemia. Czech Academy of Sciences and Faculty of Sciences. Biology Centre. Institute of Parasitology; Czech Republic.
dc.description.filFil: Nisbet, R. Ellen R. University of Cambridge. Department of Biochemistry; United Kingdom.
dc.description.filFil: Fernández Robledo, José A. Bigelow Laboratory for Ocean Sciences; United States.
dc.description.filFil: Casacuberta, Elena. Universitat Pompeu Fabra. Institut de Biologia Evolutiva; Spain.
dc.description.filFil: Sudek, Lisa. Monterey Bay Aquarium Research Institute; United States.
dc.description.filFil: Allen, Andrew E. University of California. Scripps Institution of Oceanography. Integrative Oceanography Division; United States.
dc.description.filFil: Allen, Andrew E. J. Craig Venter Institute. Microbial and Environmental Genomics; United States.
dc.description.sponsorshipGordon and Betty Moore Foundation
dc.description.sponsorshipMarine Microbiology Initiative
dc.description.sponsorshipThe Leverhulme Trust: RPG-2017-364
dc.description.sponsorshipFondos ERD: 16_019/0000759
dc.description.versionpeerreviewed
dc.format.extent481–494
dc.identifier.citationFaktorová, D., Nisbet, R.E.R., Fernández Robledo, J.A. et al. (2020). Genetic tool development in marine protists: emerging model organisms for experimental cell biology. Nat Methods, 17, 481-494. https://doi.org/10.1038/s41592-020-0796-x
dc.identifier.issn1548-7091
dc.identifier.urihttps://hdl.handle.net/2133/28847
dc.language.isoen
dc.publisherNature Research
dc.relationhttps://doi.org/10.1038/s41592-020-0828-6
dc.relation.publisherversionhttps://www.nature.com/articles/s41592-020-0796-x
dc.relation.publisherversionhttps://www.nature.com/articles/s41592-020-0796-x https://doi.org/10.1038/s41592-020-0796-x
dc.rightsopenAccess
dc.rights.holderNajle, Sebastián R.
dc.rights.holderFaktorová, Drahomíra
dc.rights.holderNisbet, R. Ellen R.
dc.rights.holderFernández Robledo, José A.
dc.rights.holderCasacuberta, Elena
dc.rights.holderSudek, Lisa
dc.rights.holderAllen, Andrew E.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectCell biology
dc.subjectUnicellular eukaryotic organisms
dc.subjectElectroporation
dc.subjectSistemas CRISPR-Cas
dc.subjectCiclo biogeoquímico
dc.titleGenetic tool development in marine protists: emerging model organisms for experimental cell biology
dc.typearticulo
dc.type.versionpublishedVersion

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