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KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells

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148.pdf (1,83 MB)

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2019-01-10

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Public Library of Science (PLOS)

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The process of neuronal differentiation is associated with neurite elongation and membrane biogenesis, and phosphatidylcholine (PtdCho) is the major membrane phospholipid in mammalian cells. During neuroblast differentiation, the transcription of two genes involved in PtdCho biosynthesis are stimulated: Chka gene for choline kinase (CK) alpha isoform and Pcyt1a gene for CTP:phosphocholine cytidylyltransferase (CCT) alpha isoform. Here we show that CKα is essential for neuronal differentiation. In addition, we demonstrated that KDM2B regulates CKα expression and, as a consequence, neuronal differentiation. This factor is up-regulated in the course of the neuroblasts proliferative and undifferentiated state and down-regulated during differentiation induced by retinoic acid (RA). During proliferation, KDM2B binds to the Box2 located in the Chka promoter repressing its transcription. Interestingly, KDM2B knockdown enhances the levels of CKα expression in neuroblast cells and induces neuronal differentiation even in the absence of RA. These results suggest that KDM2B is required for the appropriate regulation of CKα during neuronal differentiation and to the maintaining of the undifferentiated stage of neuroblast cells.
Para citar este articulo: Domizi P, Malizia F, Chazarreta-Cifre L, Diacovich L, Banchio C (2019) KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells. PLoS ONE 14(1): e0210207. https://doi.org/10.1371/journal. pone.0210207

Palabras clave

KDM2B Gene, Choline Kinase Expression, Neuronal Differentiation, Neuroblastoma Cells

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http://hdl.handle.net/2133/19669

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(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista

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Attribution 4.0 International (CC BY 4.0)
Excepto donde se indique lo contrario, la licencia de este ítem se describe como Attribution 4.0 International (CC BY 4.0)