Membrane potential assessment by fluorimetry as a predictor tool of human sperm fertilizing capacity

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dc.citation.titleFrontiers in Cell and Developmental Biology
dc.citation.volume7
dc.creatorBaró Graf, Carolina
dc.creatorRitagliati, Carla
dc.creatorTorres Monserrat, Valentina
dc.creatorStival, Cintia Estefanía
dc.creatorCarizza, Carlos
dc.creatorBuffone, Mariano Gabriel
dc.creatorKrapf, Darío
dc.date.accessioned2024-08-08T12:30:57Z
dc.date.available2024-08-08T12:30:57Z
dc.date.issued2020-01-17
dc.description.abstractMammalian sperm acquire the ability to fertilize eggs by undergoing a process known as capacitation. Capacitation is triggered as the sperm travels through the female reproductive tract. This process involves specific physiological changes such as rearrangement of the cell plasma membrane, post-translational modifications of certain proteins, and changes in the cellular permeability to ions – with the subsequent impact on the plasma membrane potential (Em). Capacitation-associated Em hyperpolarization has been well studied in mouse sperm, and shown to be both necessary and sufficient to promote the acrosome reaction (AR) and fertilize the egg. However, the relevance of the sperm Em upon capacitation on human fertility has not been thoroughly characterized. Here, we performed an extensive study of the Em change during capacitation in human sperm samples using a potentiometric dye in a fluorimetric assay. Normospermic donors showed significant Em hyperpolarization after capacitation. Em values from capacitated samples correlated significantly with the sperm ability to undergo induced AR, highlighting the role of hyperpolarization in acrosomal responsiveness, and with successful in vitro fertilization (IVF) rates. These results show that Em hyperpolarization could be an indicator of human sperm fertilizing capacity, setting the basis for the use of Em values as a robust predictor of the success rate of IVF.
dc.description.filFil: Baró Graf, Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Cell Signal Transduction Networks; Argentina.
dc.description.filFil: Baró Graf, Carolina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.filFil: Ritagliati, Carla. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Cell Signal Transduction Networks; Argentina.
dc.description.filFil: Torres Monserrat, Valentina. Grupo Oroño. Fertya medicina reproductiva; Argentina.
dc.description.filFil: Stival, Cintia Estefanía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Cell Signal Transduction Networks; Argentina.
dc.description.filFil: Carizza, Carlos. Grupo Oroño. Fertya medicina reproductiva; Argentina.
dc.description.filFil: Buffone, Mariano Gabriel. Fundación IBYME. Instituto de Biología y Medicina Experimental (IBYME-CONICET); Argentina.
dc.description.filFil: Krapf, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Laboratory of Cell Signal Transduction Networks; Argentina.
dc.description.filFil: Krapf, Darío. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2015- 3164, PICT 2017-3217
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.versionpeerreviewed
dc.format.extent1-10
dc.identifier.issn2296-634X
dc.identifier.urihttps://hdl.handle.net/2133/27549
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://doi.org/10.3389/fcell.2019.00383
dc.relation.publisherversionhttps://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2019.00383/full
dc.rightsopenAccess
dc.rights.holderBaró Graf, Carolina
dc.rights.holderRitagliati, Carla
dc.rights.holderTorres Monserrat, Valentina
dc.rights.holderStival, Cintia Estefanía
dc.rights.holderCarizza, Carlos
dc.rights.holderBuffone, Mariano Gabriel
dc.rights.holderKrapf, Darío
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMembrane potential
dc.subjectSperm capacitation
dc.subjectIn vitro fertilization
dc.subjectAcrosome reaction
dc.subjectHuman infertility
dc.titleMembrane potential assessment by fluorimetry as a predictor tool of human sperm fertilizing capacity
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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