Light modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plants

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dc.citation.titleScientific Reportses
dc.citation.volume11
dc.creatorTano, Josefina
dc.creatorRipa, María Belén
dc.creatorTondo, María Laura
dc.creatorCarrau, Analía
dc.creatorPetrocelli, Silvana
dc.creatorRodriguez, María V.
dc.creatorFerreira, Virginia
dc.creatorSiri, María I.
dc.creatorPiskulic, Laura
dc.creatorOrellano, Elena G.
dc.date.accessioned2022-03-15T12:14:53Z
dc.date.available2022-03-15T12:14:53Z
dc.date.issued2021-07-15
dc.descriptionRalstonia pseudosolanacearum GMI1000 (Rpso GMI1000) is a soil-borne vascular phytopathogen that infects host plants through the root system causing wilting disease in a wide range of agroeconomic interest crops, producing economical losses. Several features contribute to the full bacterial virulence. In this work we study the participation of light, an important environmental factor, in the regulation of the physiological attributes and infectivity of Rpso GMI1000. In silico analysis of the Rpso genome revealed the presence of a Rsp0254 gene, which encodes a putative blue light LOV-type photoreceptor. We constructed a mutant strain of Rpso lacking the LOV protein and found that the loss of this protein and light, infuenced characteristics involved in the pathogenicity process such as motility, adhesion and the bioflms development, which allows the successful host plant colonization, rendering bacterial wilt. This protein could be involved in the adaptive responses to environmental changes. We demonstrated that light sensing and the LOV protein, would be used as a location signal in the host plant, to regulate the expression of several virulence factors, in a time and tissue dependent way. Consequently, bacteria could use an external signal and Rpsolov gene to know their location within plant tissue during the colonization process.es
dc.description.filFil: Tano, Josefina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Ripa, María Belén. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Carrau, Analía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Petrocelli, Silvana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es
dc.description.filFil: Rodriguez, María V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Ferreira, Virginia. Universidad de la República. Facultad de Química. Departamento de Biociencias. Área Microbiología; Uruguay.es
dc.description.filFil: Siri, María I. Universidad de la República. Facultad de Química. Departamento de Biociencias. Área Microbiología; Uruguay.es
dc.description.filFil: Piskulic, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Estadística y Procesamiento de datos; Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipUniversidad Nacional de Rosario (UNR): Grant N° 1BIO432
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT2017-2242 to E.G.O.
dc.formatapplication/pdf
dc.format.extent1-17es
dc.identifier.issn2045-2322es
dc.identifier.urihttp://hdl.handle.net/2133/23188
dc.language.isoenges
dc.publisherNature Researches
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-021-93871-9
dc.relation.publisherversionhttps://www.nature.com/articles/s41598-021-93871-9#:~:text=We%20demonstrated%20that%20light%20and,rendering%20the%20bacterial%20wilt%20disease.
dc.rightsopenAccesses
dc.rights.holderTano, Josefinaes
dc.rights.holderRipa, María Belénes
dc.rights.holderTondo, María Lauraes
dc.rights.holderCarrau, Analíaes
dc.rights.holderPetrocelli, Silvanaes
dc.rights.holderRodriguez, María V.es
dc.rights.holderFerreira, Virginiaes
dc.rights.holderSiri, María I.es
dc.rights.holderPiskulic, Lauraes
dc.rights.holderOrellano, Elena G.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectRalstoniaes
dc.subjectBacterial Adhesiones
dc.subjectBacterial Proteinses
dc.subjectLightes
dc.subjectMetabolismes
dc.titleLight modulates important physiological features of Ralstonia pseudosolanacearum during the colonization of tomato plantses
dc.typepublishedVersion
dc.type.versionpublishedVersion

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