An industrial scale process for the enzymatic removal of steryl glucosides from biodiesel

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dc.citation.titleBiotechnology for Biofuels
dc.citation.volume8
dc.creatorPeirú, Salvador
dc.creatorAguirre, Andrés
dc.creatorEberhardt, María Florencia
dc.creatorBraia, Mauricio Javier
dc.creatorCabrera, Rodolfo
dc.creatorMenzella, Hugo G.
dc.date.accessioned2024-09-12T14:57:29Z
dc.date.available2024-09-12T14:57:29Z
dc.date.issued2015-12-21
dc.description.abstractBackground: Biodiesels produced from transesterification of vegetable oils have a major quality problem due to the presence of precipitates, which need to be removed to avoid clogging of filters and engine failures. These precipitates have been reported to be mostly composed of steryl glucosides (SGs), but so far industrial cost-effective methods to remove these compounds are not available. Here we describe a novel method for the efficient removal of SGs from biodiesel, based on the hydrolytic activity of a thermostable β-glycosidase obtained from Thermococcus litoralis. Results: A steryl glucosidase (SGase) enzyme from T. litoralis was produced and purified from Escherichia coli cultures expressing a synthetic gene, and used to treat soybean-derived biodiesel. Several optimization steps allowed for the selection of optimal reaction conditions to finally provide a simple and efficient process for the removal of SGs from crude biodiesel. The resulting biodiesel displayed filterability properties similar to distilled biodiesel according to the total contamination (TC), the cold soak filtration test (CSFT), filter blocking tendency (FBT), and cold soak filter blocking tendency (CSFBT) tests. The process was successfully scaled up to a 20 ton reactor, confirming its adaptability to industrial settings. Conclusions: The results presented in this work provide a novel path for the removal of steryl glucosides from biodiesel using a cost-effective, environmentally friendly and scalable enzymatic process, contributing to the adoption of this renewable fuel.
dc.description.filFil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology (CONICET); Argentina.
dc.description.filFil: Peirú, Salvador. Keclon S.A.; Argentina.
dc.description.filFil: Aguirre, Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology (CONICET); Argentina.
dc.description.filFil: Aguirre, Andrés. Keclon S.A.; Argentina.
dc.description.filFil: Eberhardt, María Florencia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology (CONICET); Argentina.
dc.description.filFil: Braia, Mauricio Javier. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology (CONICET); Argentina.
dc.description.filFil: Cabrera, Rodolfo. Unitec Bio S.A.; Argentina.
dc.description.filFil: Menzella, Hugo G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology (CONICET); Argentina.
dc.description.filFil: Menzella, Hugo G. Keclon S.A.; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2010-1157
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.versionpeerreviewed
dc.format.extent1-12
dc.identifier.issn1754-6834
dc.identifier.issn2731-3654
dc.identifier.urihttps://hdl.handle.net/2133/27788
dc.language.isoen
dc.publisherBMC (part of Springer Nature)
dc.relation.publisherversionhttps://doi.org/10.1186/s13068-015-0405-x
dc.relation.publisherversionhttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0405-x
dc.rightsopenAccess
dc.rights.holderPeirú, Salvador
dc.rights.holderAguirre, Andrés
dc.rights.holderEberhardt, María Florencia
dc.rights.holderBraia, Mauricio Javier
dc.rights.holderCabrera, Rodolfo
dc.rights.holderMenzella, Hugo G.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSynthetic biology
dc.subjectGreen chemistry
dc.subjectBiofuels
dc.subjectSteryl glucosides (SGs)
dc.subjectβ-glycosidase
dc.subjectThermococcus
dc.titleAn industrial scale process for the enzymatic removal of steryl glucosides from biodiesel
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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