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Fibroblast growth factor receptors (FGFRs) in human sperm: expression, functionality and involvement in motility regulation

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dc.citation.titlePLoS ONE
dc.citation.volume10
dc.contributor.otherDr. Baldi, Alberto provide the FGF2
dc.creatorSaucedo, Lucía
dc.creatorBuffa, Gabriela Natalia
dc.creatorRosso, Marina
dc.creatorGuillardoy, Tomás
dc.creatorGóngora, Adrián
dc.creatorMunuce, María José
dc.creatorVázquez Levin, Mónica Hebe
dc.creatorMarín Briggiler, Clara
dc.date.accessioned2024-10-08T14:03:25Z
dc.date.available2024-10-08T14:03:25Z
dc.date.issued2015-05-13
dc.description.abstractFibroblast growth factors receptors (FGFRs) have been widely characterized in somatic cells, but there is scarce evidence of their expression and function in mammalian gametes. The objective of the present study was to evaluate the expression of FGFRs in human male germ cells, to determine sperm FGFR activation by the FGF2 ligand and their participation in the regulation of sperm motility. The expression of FGFR1, 2, 3 and 4 mRNAs and proteins in human testis and localization of these receptors in germ cells of the seminiferous epithelium was demonstrated. In ejaculated sperm, FGFRs were localized to the acrosomal region and flagellum. Sperm exposure to FGF2 caused an increase in flagellar FGFR phosphorylation and activation of extracellular signal-regulated kinase (ERK) and protein kinase B (PKB or Akt) signaling pathways. Incubation with FGF2 led to a significant increase in the percentage of total and progressive sperm motility, as well as in sperm kinematics. All responses were prevented by sperm preincubation with BGJ398, a specific inhibitor of FGFR tyrosine kinase activity. In addition to confirming the expression of FGFRs in germ cells of the human testis, our study describes for the first time the presence, localization and functionality of human sperm FGFRs, and provides evidence of the beneficial effect of FGF2 upon sperm motility.
dc.description.filFil: Saucedo, Lucía. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Buffa, Gabriela Natalia. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Rosso, Marina. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Guillardoy, Tomás. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Góngora, Adrián. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Munuce, María José. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área de Bioquímica Clínica. Laboratorio de Medicina Reproductiva; Argentina.
dc.description.filFil: Munuce, María José. Sanatorio Británico de Rosario. Repro Lab Inc.; Argentina.
dc.description.filFil: Vázquez Levin, Mónica Hebe. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.filFil: Marín Briggiler, Clara. Instituto de Biología y Medicina Experimental (IBYME-CONICET/Fundación IBYME); Argentina.
dc.description.sponsorshipFundación Florencio Fiorini
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2013-627
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 2008-2120, PIP 2012-740
dc.description.sponsorshipRepro Lab Inc.
dc.description.versionpeerreviewed
dc.format.extent1-17
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2133/27941
dc.language.isoen
dc.publisherPublic Library of Science (PLOS)
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0127297
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0127297
dc.rightsopenAccess
dc.rights.holderSaucedo, Lucía
dc.rights.holderBuffa, Gabriela Natalia
dc.rights.holderRosso, Marina
dc.rights.holderGuillardoy, Tomás
dc.rights.holderGóngora, Adrián
dc.rights.holderMunuce, María José
dc.rights.holderVázquez Levin, Mónica Hebe
dc.rights.holderMarín Briggiler, Clara
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectReceptors, Fibroblast Growth Factor
dc.subjectFGFRs
dc.subjectFibroblast Growth Factors
dc.subjectFGF
dc.subjectFibroblast Growth Factor 2
dc.subjectFGF2
dc.subjectSpermatozoa
dc.subjectSperm
dc.subjectSperm motility
dc.titleFibroblast growth factor receptors (FGFRs) in human sperm: expression, functionality and involvement in motility regulation
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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