Acinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanisms

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dc.citation.titlemSphere
dc.citation.volume5
dc.creatorRepizo, Guillermo Daniel
dc.creatorEspariz, Martín
dc.creatorSeravalle, Joana L.
dc.creatorDíaz Miloslavich, Juan Ignacio
dc.creatorSteimbrüch, Bruno A.
dc.creatorShuman, Howard A.
dc.creatorViale, Alejandro M.
dc.date.accessioned2021-04-20T16:44:56Z
dc.date.available2021-04-20T16:44:56Z
dc.date.issued2020-07-29
dc.descriptionAcinetobacter baumannii represents nowadays an important nosocomial pathogen of poorly defined reservoirs outside the clinical setting. Here, we con ducted whole-genome sequencing analysis of the Acinetobacter sp. NCIMB8209 col lection strain, isolated in 1943 from the aerobic degradation (retting) of desert gu ayule shrubs. Strain NCIMB8209 contained a 3.75-Mb chromosome and a plasmid of 134 kb. Phylogenetic analysis based on core genes indicated NCIMB8209 affiliation to A. baumannii, a result supported by the identification of a chromosomal blaOXA 51-like gene. Seven genomic islands lacking antimicrobial resistance determinants, 5 regions encompassing phage-related genes, and notably, 93 insertion sequences (IS) were found in this genome. NCIMB8209 harbors most genes linked to persistence and virulence described in contemporary A. baumannii clinical strains, but many of the genes encoding components of surface structures are interrupted by IS. More over, defense genetic islands against biological aggressors such as type 6 secretion systems or CRISPR-cas are absent from this genome. These findings correlate with a low capacity of NCIMB8209 to form biofilm and pellicle, low motility on semisolid medium, and low virulence toward Galleria mellonella and Caenorhabditis elegans. Searching for catabolic genes and concomitant metabolic assays revealed the ability of NCIMB8209 to grow on a wide range of substances produced by plants, including aromatic acids and defense compounds against external aggressors. All the above features strongly suggest that NCIMB8209 has evolved specific adaptive features to a particular environmental niche. Moreover, they also revealed that the remarkable genetic plasticity identified in contemporary A. baumannii clinical strains represents an intrinsic characteristic of the species.es
dc.description.filFil: Repizo, Guillermo Daniel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Repizo, Guillermo Daniel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Espariz, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Seravalle, Joana L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Seravalle, Joana L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Díaz Miloslavich, Juan Ignacio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Díaz Miloslavich, Juan Ignacio. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Steimbrüch, Bruno A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Steimbrüch, Bruno A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.filFil: Shuman, Howard A. University of Chicago. Department of Microbiology; United States.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.
dc.description.filFil: Viale, Alejandro M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Microbiología; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2015-1072es
dc.description.sponsorshipNational Institutes of Health (NIH): AI-115203es
dc.formatapplication/pdf
dc.format.extent1-21
dc.identifier.issn2379-5042
dc.identifier.urihttp://hdl.handle.net/2133/20524
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.publisherversionhttps://doi.org/10.1128/MSPHERE.00404-20es
dc.relation.publisherversionhttps://msphere.asm.org/content/5/4/e00404-20es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderRepizo, Guillermo Danieles
dc.rights.holderEspariz, Martínes
dc.rights.holderSeravalle, Joana L.es
dc.rights.holderDíaz Miloslavich, Juan Ignacioes
dc.rights.holderSteimbrüch, Bruno A.es
dc.rights.holderShuman, Howard A.es
dc.rights.holderViale, Alejandro M.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectAcinetobacter baumanniies
dc.subjectGenomicses
dc.subjectInsertion Sequenceses
dc.subjectVirulence Factorses
dc.subjectPre-antibiotic Eraes
dc.subjectEnvironmental Reservoirses
dc.titleAcinetobacter baumannii NCIMB8209: a rare environmental strain displaying extensive insertion sequence-mediated genome remodeling resulting in the loss of exposed cell structures and defensive mechanismses
dc.typeartículo
dc.typearticle
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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