Recombinant protein expression in Escherichia coli: advances and challenges

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dc.citation.titleFrontiers in Microbiology
dc.citation.volume1-17
dc.creatorRosano, Germán L.
dc.creatorCeccarelli, Eduardo Augusto
dc.date.accessioned2024-03-18T14:08:20Z
dc.date.available2024-03-18T14:08:20Z
dc.date.issued2014-04-17
dc.description.abstractEscherichia coli is one of the organisms of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform. For this reason, there are many molecular tools and protocols at hand for the high-level production of heterologous proteins, such as a vast catalog of expression plasmids, a great number of engineered strains and many cultivation strategies.We review the different approaches for the synthesis of recombinant proteins in E. coli and discuss recent progress in this ever-growing field.
dc.description.filFil: Rosano, Germán L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Rosano, Germán L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.description.filFil: Ceccarelli, Eduardo Augusto. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ceccarelli, Eduardo Augusto. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/2133/26795
dc.language.isoen
dc.publisherFrontiers Media
dc.rightsopenAccess
dc.rights.holderRosano, Germán L.
dc.rights.holderCeccarelli, Eduardo Augusto
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectRecombinant protein expression
dc.subjectEscherichia coli
dc.subjectExpression plasmid
dc.subjectInclusion bodies
dc.subjectAffinity tags
dc.subjectE. coli expression strains
dc.titleRecombinant protein expression in Escherichia coli: advances and challenges
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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