Plastid-targeted Cyanobacterial Flavodiiron proteins maintain carbohydrate turnover and enhance drought stress tolerance in barley

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dc.citation.titleFrontiers in Plant Science
dc.citation.volume11
dc.creatorShahinnia, Fahimeh
dc.creatorTula, Suresh
dc.creatorHensel, Goetz
dc.creatorReiahisamani, Narges
dc.creatorNasr, Nasrin
dc.creatorKumlehn, Jochen
dc.creatorGómez, Rodrigo Lionel
dc.creatorLodeyro, Anabella F.
dc.creatorCarrillo, Néstor
dc.creatorHajirezaei, Mohammad-Reza
dc.date.accessioned2024-05-10T13:59:53Z
dc.date.available2024-05-10T13:59:53Z
dc.date.issued2021-02-13
dc.description.abstractChloroplasts, the sites of photosynthesis in higher plants, have evolved several means to tolerate short episodes of drought stress through biosynthesis of diverse metabolites essential for plant function, but these become ineffective when the duration of the stress is prolonged. Cyanobacteria are the closest bacterial homologs of plastids with two photosystems to perform photosynthesis and to evolve oxygen as a byproduct. The presence of Flv genes encoding flavodiiron proteins has been shown to enhance stress tolerance in cyanobacteria. In an attempt to support the growth of plants exposed to drought, the Synechocystis genes Flv1 and Flv3 were expressed in barley with their products being targeted to the chloroplasts. The heterologous expression of both Flv1 and Flv3 accelerated days to heading, increased biomass, promoted the number of spikes and grains per plant, and improved the total grain weight per plant of transgenic lines exposed to drought. Improved growth correlated with enhanced availability of soluble sugars, a higher turnover of amino acids and the accumulation of lower levels of proline in the leaf. Flv1 and Flv3 maintained the energy status of the leaves in the stressed plants by converting sucrose to glucose and fructose, immediate precursors for energy production to support plant growth under drought. The results suggest that sugars and amino acids play a fundamental role in the maintenance of the energy status and metabolic activity to ensure growth and survival under stress conditions, that is, water limitation in this particular case. Engineering chloroplasts by Flv genes into the plant genome, therefore, has the potential to improve plant productivity wherever drought stress represents a significant production constraint.
dc.description.filFil: Shahinnia, Fahimeh. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Tula, Suresh. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Hensel, Goetz. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Hensel, Goetz. Palacký University. Faculty of Science. Centre of the Region Hana for Biotechnological and Agriculture Research. Division of Molecular Biology; Czechia.
dc.description.filFil: Reiahisamani, Narges. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Nasr, Nasrin. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Nasr, Nasrin. Payame Noor University. Department of Biology; Iran.
dc.description.filFil: Kumlehn, Jochen. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.filFil: Gómez, Rodrigo Lionel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Lodeyro, Anabella F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (CONICET-IBR); Argentina.
dc.description.filFil: Hajirezaei, Mohammad-Reza. Institute of Plant Genetics and Crop Plant Research. Department of Physiology and Cell Biology: Germany.
dc.description.sponsorshipBundesministerium für Bildung und Forschung (BMBF): PICT 2015-3828
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2017-3080
dc.description.sponsorshipAgencia Santafesina de Ciencia Tecnología e Innovación: IO 212-2017
dc.format.extent1-15
dc.identifier.issn1664-462X
dc.identifier.urihttps://hdl.handle.net/2133/27016
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.613731/full
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2020.613731
dc.rightsopenAccess
dc.rights.holderShahinnia, Fahimeh
dc.rights.holderTula, Suresh
dc.rights.holderReiahisamani, Narges
dc.rights.holderNasr, Nasrin
dc.rights.holderKumlehn, Jochen
dc.rights.holderGómez, Rodrigo Lionel
dc.rights.holderLodeyro, Anabella F.
dc.rights.holderCarrillo, Néstor
dc.rights.holderHajirezaei, Mohammad-Reza
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectBiomass
dc.subjectHordeum vulgare L
dc.subjectMetabolites
dc.subjectPhotosynthesis
dc.subjectPlastid biotechnology
dc.subjectYield
dc.titlePlastid-targeted Cyanobacterial Flavodiiron proteins maintain carbohydrate turnover and enhance drought stress tolerance in barley
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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