Expression of codon optimized genes in microbial systems: current industrial applications and perspectives

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dc.citation.titleFrontiers in Microbiology
dc.citation.volume1-8
dc.citation.volume5
dc.creatorElena, Claudia
dc.creatorRavasi, Pablo
dc.creatorCastelli, María Eugenia
dc.creatorPeirú, Salvador
dc.creatorMenzella
dc.date.accessioned2024-04-12T16:43:27Z
dc.date.available2024-04-12T16:43:27Z
dc.date.issued2014-02-14
dc.description.abstractThe efficient production of functional proteins in heterologous hosts is one of the major bases of modern biotechnology. Unfortunately, many genes are difficult to express outside their original context. Due to their apparent “silent” nature, synonymous codon substitutions have long been thought to be trivial. In recent years, this dogma has been refuted by evidence that codon replacement can have a significant impact on gene expression levels and protein folding. In the past decade, considerable advances in the speed and cost of gene synthesis have facilitated the complete redesign of entire gene sequences, dramatically improving the likelihood of high protein expression. This technology significantly impacts the economic feasibility of microbial-based biotechnological processes by, for example, increasing the volumetric productivities of recombinant proteins or facilitating the redesign of novel biosynthetic routes for the production of metabolites. This review discusses the current applications of this technology, particularly those regarding the production of small molecules and industrially relevant recombinant enzymes. Suggestions for future research and potential uses are provided as well.
dc.description.filFil: Elena, Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology; Argentina.
dc.description.filFil: Ravasi, Pablo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology; Argentina.
dc.description.filFil: Castelli, María Eugenia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology; Argentina.
dc.description.filFil: Peirú, Salvador. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology; Argentina.
dc.description.filFil: Menzella, Hugo G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Genetic Engineering and Fermentation Technology; Argentina.
dc.identifier.issn1664-302X
dc.identifier.urihttps://hdl.handle.net/2133/26874
dc.language.isoen
dc.publisherFrontiers Media
dc.relation.publisherversionhttps://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2014.00021/full#h3
dc.relation.publisherversionhttps://doi.org/10.3389/fmicb.2014.00021
dc.rightsopenAccess
dc.rights.holderElena, Claudia
dc.rights.holderRavasi, Pablo
dc.rights.holderCastelli, María Eugenia
dc.rights.holderPeirú, Salvador
dc.rights.holderMenzella, Hugo G.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectSynthetic biology
dc.subjectGene design
dc.subjectCodon optimization
dc.subjectStrain engineering
dc.subjectMicrobial systems
dc.titleExpression of codon optimized genes in microbial systems: current industrial applications and perspectives
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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