Identification of inhibitors targeting ferredoxin-NADP+ reductase from the Xanthomonas citri subsp. citri phytopathogenic bacteria

Vista sencilla de ítem
dc.citation.titleMoleculeses
dc.citation.volume23(1)es
dc.creatorMartínez-Júlvez, Marta
dc.creatorGoñi, Guillermina
dc.creatorPérez-Amigot, Daniel
dc.creatorLaplaza, Rubén
dc.creatorIonescu, Irina Alexandra
dc.creatorPetrocelli, Silvana
dc.creatorTondo, María Laura
dc.creatorSancho, Javier
dc.creatorOrellano, Elena G.
dc.creatorMedina, Milagros
dc.date.accessioned2021-02-22T17:58:05Z
dc.date.available2021-02-22T17:58:05Z
dc.date.issued2017-12-24
dc.descriptionFerredoxin-NADP(H) reductases (FNRs) deliver NADPH or low potential one-electron donors to redox-based metabolism in plastids and bacteria. Xanthomonas citri subsp. citri (Xcc) is a Gram-negative bacterium responsible for citrus canker disease that affects commercial citrus crops worldwide. The Xcc fpr gene encodes a bacterial type FNR (XccFPR) that contributes to the bacterial response to oxidative stress conditions, usually found during plant colonization. Therefore, XccFPR is relevant for the pathogen survival and its inhibition might represent a strategy to treat citrus canker. Because of mechanistic and structural differences from plastidic FNRs, XccFPR is also a potential antibacterial target. We have optimized an activity-based high-throughput screening (HTS) assay that identifies XccFPR inhibitors. We selected 43 hits from a chemical library and narrowed them down to the four most promising inhibitors. The antimicrobial effect of these compounds was evaluated on Xcc cultures, finding one with antimicrobial properties. Based on the functional groups of this compound and their geometric arrangement, we identified another three XccFPR inhibitors. Inhibition mechanisms and constants were determined for these four XccFPR inhibitors. Their specificity was also evaluated by studying their effect on the plastidic Anabaena PCC 7119 FNR, finding differences that can become interesting tools to discover Xcc antimicrobials.es
dc.descriptionPara citar este articulo: Martínez-Júlvez, M.; Goñi, G.; Pérez-Amigot, D.; Laplaza, R.; Ionescu, I.A.; Petrocelli, S.; Tondo, M.L.; Sancho, J.; Orellano, E.G.; Medina, M. Identification of Inhibitors Targeting Ferredoxin-NADP+ Reductase from the Xanthomonas citri subsp. citri Phytopathogenic Bacteria. Molecules 2018, 23, 29. https://doi.org/10.3390/molecules23010029
dc.description.filFil: Martínez-Júlvez, Marta. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Goñi, Guillermina. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Pérez-Amigot, Daniel. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Laplaza, Rubén. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Laplaza, Rubén. Universidad de Santiago de Compostela. Departamento de Química Física; España.es
dc.description.filFil: Ionescu, Irina Alexandra. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Petrocelli, Silvana. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Tondo, María Laura. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Sancho, Javier. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.filFil: Orellano, Elena G. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Medina, Milagros. Universidad de Zaragoza. Facultad de Ciencias e Institute of Biocomputation and Physics of Complex Systems (BIFI-IQFR and CBsC-CSIC Joint Units). Departamento de Bioquímica y Biología Molecular y Celular; España.es
dc.description.sponsorshipMinisterio de Economía Industria y Competitividad (MINECO): BIO2016-75183-P AEI/FEDER, UE y BFU 2016-78232-Pes
dc.description.sponsorshipGobierno de Aragón: FEDER [B18]es
dc.formatapplication/pdf
dc.format.extent1-15es
dc.identifier.issn1420-3049es
dc.identifier.urihttp://hdl.handle.net/2133/19666
dc.language.isoenges
dc.publisherMDPIes
dc.relation.publisherversionhttps://doi.org/10.3390/molecules23010029es
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/23/1/29es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderMartínez-Júlvez, Martaes
dc.rights.holderGoñi, Guillerminaes
dc.rights.holderPérez-Amigot, Danieles
dc.rights.holderLaplaza, Rubénes
dc.rights.holderIonescu, Irina Alexandraes
dc.rights.holderPetrocelli, Silvanaes
dc.rights.holderTondo, María Lauraes
dc.rights.holderSancho, Javieres
dc.rights.holderOrellano, Elena G.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectActivity-Based High-Throughput Screening;es
dc.subjectEnzyme Inhibitorses
dc.subjectFerredoxin-NADP Reductasees
dc.subjectXanthomonas citri subsp. citries
dc.titleIdentification of inhibitors targeting ferredoxin-NADP+ reductase from the Xanthomonas citri subsp. citri phytopathogenic bacteriaes
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
336.pdf
Tamaño:
3.66 MB
Formato:
Adobe Portable Document Format
Descripción:
versión post-print
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.59 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista