Expression of Flavodiiron Proteins Flv2-Flv4 in Chloroplasts of Arabidopsis and Tobacco Plants Provides Multiple Stress Tolerance

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dc.citation.titleInternational Journal of Molecular Sciences
dc.citation.volume22
dc.contributor.orcidhttps://orcid.org/0000-0002-8789-0899
dc.contributor.orcidhttps://orcid.org/0000-0001-8236-7647
dc.contributor.orcidhttps://orcid.org/0000-0002-5213-4030
dc.creatorVicino, Paula
dc.creatorCarrillo, Julieta
dc.creatorGómez, Rodrigo Lionel
dc.creatorShahinnia, Fahimeh
dc.creatorTula, Suresh
dc.creatorMelzer, Michael
dc.creatorRutten, Twan
dc.creatorCarrillo, Néstor
dc.creatorHajirezaei, Mohammad-Reza
dc.creatorLodeyro, Anabella F.
dc.date.accessioned2024-11-11T15:15:26Z
dc.date.available2024-11-11T15:15:26Z
dc.date.issued2021-01-25
dc.description.abstractWith the notable exception of angiosperms, all phototrophs contain different sets of flavodiiron proteins that help to relieve the excess of excitation energy on the photosynthetic electron transport chain during adverse environmental conditions, presumably by reducing oxygen directly to water. Among them, the Flv2-Flv4 dimer is only found in β-cyanobacteria and induced by high light, supporting a role in stress protection. The possibility of a similar protective function in plants was assayed by expressing Synechocystis Flv2-Flv4 in chloroplasts of tobacco and Arabidopsis. Flv-expressing plants exhibited increased tolerance toward high irradiation, salinity, oxidants, and drought. Stress tolerance was reflected by better growth, preservation of photosynthetic activity, and membrane integrity. Metabolic profiling under drought showed enhanced accumulation of soluble sugars and amino acids in transgenic Arabidopsis and a remarkable shift of sucrose into starch, in line with metabolic responses of drought-tolerant genotypes. Our results indicate that the Flv2-Flv4 complex retains its stress protection activities when expressed in chloroplasts of angiosperm species by acting as an additional electron sink. The flv2-flv4 genes constitute a novel biotechnological tool to generate plants with increased tolerance to agronomically relevant stress conditions that represent a significant productivity constraint.
dc.description.filFil: Vicino, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Carrillo, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gómez, Rodrigo Lionel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Shahinnia, Fahimeh. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Tula, Suresh. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Melzer, Michael. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Rutten, Twan. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Carrillo, Néstor. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Hajirezaei, Mohammad-Reza. Leibniz Institute of Plant Genetics and Crop Plant Research; Germany.
dc.description.filFil: Lodeyro, Anabella F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipDeutscher Akademischer Austauschdienst (DAAD)
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)
dc.description.sponsorshipFederal Ministry of Education and Research (BMBF): FKZ 031A280
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (ANPCyT): PICT 2015-3828, PICT 2017-308
dc.description.sponsorshipAgencia Santafesina de Ciencia Tecnología e Innovación: IO 212-2017
dc.format.extent1-21
dc.identifier.citationVicino, Paula; Carrillo, Julieta Beatriz; Gomez, Rodrigo Lionel; Shahinnia, Fahimeh; Tula, Suresh; et al.; Expression of Flavodiiron Proteins Flv2-Flv4 in Chloroplasts of Arabidopsis and Tobacco Plants Provides Multiple Stress Tolerance; MDPI AG; International Journal of Molecular Sciences; 22; 3; 2-2021; 1-21
dc.identifier.issn1661-6596
dc.identifier.urihttps://hdl.handle.net/2133/28091
dc.language.isoen
dc.publisherMDPI
dc.relation.publisherversionhttps://doi.org/10.3390/ijms22031178
dc.relation.publisherversionhttps://www.mdpi.com/1422-0067/22/3/1178
dc.rightsopenAccess
dc.rights.holderVicino, Paula
dc.rights.holderCarrillo, Julieta
dc.rights.holderGómez, Rodrigo Lionel
dc.rights.holderShahinnia, Fahimeh
dc.rights.holderTula, Suresh
dc.rights.holderMelzer, Michael
dc.rights.holderRutten, Twan
dc.rights.holderCarrillo, Néstor
dc.rights.holderHajirezaei, Mohammad-Reza
dc.rights.holderLodeyro, Anabella F.
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFlavodiiron 2-4
dc.subjectHigh light
dc.subjectDrought
dc.subjectStress tolerance
dc.subjectNicotiana tabacum
dc.subjectArabidopsis thaliana
dc.subjectSynechocystis
dc.titleExpression of Flavodiiron Proteins Flv2-Flv4 in Chloroplasts of Arabidopsis and Tobacco Plants Provides Multiple Stress Tolerance
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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