High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties

Vista sencilla de ítem
dc.citation.titleBiotechnology for Biofuels
dc.citation.volume9
dc.creatorMenendez Bravo, Simón M.
dc.creatorRoulet, Julia
dc.creatorSabatini, Martín
dc.creatorComba, Santiago
dc.creatorDunn, Robert
dc.creatorGramajo, Hugo Cesar
dc.creatorArabolaza, Ana Lorena
dc.date.accessioned2024-10-02T12:45:45Z
dc.date.available2024-10-02T12:45:45Z
dc.date.issued2016-10-14
dc.description.abstractBackground: microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety of usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage of a polyketide synthase-based system from Mycobacterium tuberculosis and developed an Escherichia coli platform with the capacity to synthesize multimethyl-branched long-chain esters (MBE) with novel chemical structures. Results: with the aim to initiate the characterization of these novel waxy compounds, here, we describe the chassis optimization of the MBE producer E. coli strain for an up-scaled oil production. By carrying out systematic metabolic engineering, we improved the final titer to 138.1 ± 5.3 mg MBE L−1 in batch cultures. Fed-batch microbial fermentation process was also optimized achieving a maximum yield of 790.2 ± 6.9 mg MBE L−1 with a volumetric productivity of 15.8 ± 1.1 mg MBE (L h)−1. Purified MBE oil was subjected to various physicochemical analyses, including differential scanning calorimetry (DSC) and pressurized-differential scanning calorimetry (P-DSC) studies. Conclusions: the analysis of the pour point, DSC, and P-DSC data obtained showed that bacterial MBE possess improved cold flow properties than several plant oils and some chemically modified derivatives, while exhibiting high oxidation stability at elevated temperatures. These encouraging data indicate that the presence of multiple methyl branches in these novel esters, indeed, conferred favorable properties which are superior to those of linear esters.
dc.description.filFil: Menendez Bravo, Simón M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Roulet, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Sabatini, Martín. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Comba, Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Dunn, Robert. National Center for Agricultural Utilization Research. Agricultural Research Service. U.S. Department of Agriculture; United States.
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.filFil: Arabolaza, Ana Lorena. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET). Departamento de Microbiología; Argentina.
dc.description.sponsorshipYPF Tecnología S.A. (Y-TEC - YPF/CONICET). Programa de Biotecnología Industrial de YPF-Tecnología: grant PID 2013-0042
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2013-1981
dc.description.versionpeerreviewed
dc.format.extent1-14
dc.identifier.issn1754-6834
dc.identifier.urihttps://hdl.handle.net/2133/27909
dc.language.isoen
dc.publisherBMC (part of Springer Nature)
dc.relation.publisherversionhttps://doi.org/10.1186/s13068-016-0631-x
dc.relation.publisherversionhttps://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0631-x
dc.rightsopenAccess
dc.rights.holderMenendez Bravo, Simón M.
dc.rights.holderRoulet, Julia
dc.rights.holderSabatini, Martín
dc.rights.holderComba, Santiago
dc.rights.holderDunn, Robert
dc.rights.holderGramajo, Hugo Cesar
dc.rights.holderArabolaza, Ana Lorena
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectEscherichia coli
dc.subjectWax production
dc.subjectMetabolic engineering
dc.subjectFed-batch fermentation
dc.subjectOleochemicals
dc.titleHigh cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties
dc.typearticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
File - s13068-016-0631-x.pdf
Tamaño:
1.22 MB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Microbiología - Artículos
(FBIOyF) Departamento de Química Orgánica - Artículos