Interspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infection

Vista sencilla de ítem
dc.citation.titleFrontiers in Plant Sciencees
dc.citation.volume8es
dc.creatorFerreira, Virginia
dc.creatorPianzzola, María J.
dc.creatorVilaró, Francisco L.
dc.creatorGalván, Guillermo A.
dc.creatorTondo, María Laura
dc.creatorRodriguez, María V.
dc.creatorOrellano, Elena G.
dc.creatorValls, Marc
dc.date.accessioned2021-02-19T19:29:30Z
dc.date.available2021-02-19T19:29:30Z
dc.date.issued2017-08-28
dc.descriptionPotato (Solanum tuberosum L.) is one of the main hosts of Ralstonia solanacearum, the causative agent of bacterial wilt. This plant pathogen bacteria produce asymptomatic latent infections that promote its global spread, hindering disease control. A potato breeding program is conducted in Uruguay based on the introgression of resistance from the wild native species S. commersonii Dun. Currently, several backcrosses were generated exploiting the high genetic variability of this wild species resulting in advanced interspecific breeding lines with different levels of bacterial wilt resistance. The overall aim of this work was to characterize the interaction of the improved potato germplasm with R. solanacearum. Potato clones with different responses to R. solanacearum were selected, and colonization, dissemination and multiplication patterns after infection were evaluated. A R. solanacearum strain belonging to the phylotype IIB-sequevar 1, with high aggressiveness on potato was genetically modified to constitutively generate fluorescence and luminescence from either the green fluorescence protein gene or lux operon. These reporter strains were used to allow a direct and precise visualization of fluorescent and luminescent cells in plant tissues by confocal microscopy and luminometry. Based on wilting scoring and detection of latent infections, the selected clones were classified as susceptible or tolerant, while no immune-like resistance response was identified. Typical wilting symptoms in susceptible plants were correlated with high concentrations of bacteria in roots and along the stems. Tolerant clones showed a colonization pattern restricted to roots and a limited number of xylem vessels only in the stem base. Results indicate that resistance in potato is achieved through restriction of bacterial invasion and multiplication inside plant tissues, particularly in stems. Tolerant plants were also characterized by induction of anatomical and biochemical changes after R. solanacearum infection, including hyperplasic activity of conductor tissue, tylose production, callose and lignin deposition, and accumulation of reactive oxygen species. This study highlights the potential of the identified tolerant interspecific potato clones as valuable genetic resources for potato-breeding programs and leads to a better understanding of resistance against R. solanacearum in potato.es
dc.description.filFil: Ferreira, Virginia. Universidad de la República. Facultad de Química. Departamento de Biociencias; Uruguay.es
dc.description.filFil: Pianzzola, María J. Universidad de la República. Facultad de Química. Departamento de Biociencias; Uruguay.es
dc.description.filFil: Vilaró, Francisco L. Instituto Nacional de Investigación Agropecuaria (INIA). Unidad de Horticultura; Uruguay.es
dc.description.filFil: Galván, Guillermo A. Universidad de la República. Facultad de Agronomía. Centro Regional Sur. Departamento de Producción Vegetal; Uruguay.es
dc.description.filFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Farmacia y Bioquímica. Área Biología Molecular; Argentina.es
dc.description.filFil: Rodriguez, María V. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Vegetal; Argentina.es
dc.description.filFil: Rodriguez, María V. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Farmacia y Bioquímica. Área Biología Molecular; Argentina.es
dc.description.filFil: Valls, Marc. Consorcio CSIC-IRTA-UAB-UB Centro de Investigación Agrogenómica (CRAG); España.es
dc.description.filFil: Valls, Marc. Universitat de Barcelona. Department of Genetics; Spain.es
dc.description.sponsorshipPrograma de Desarrollo de las Ciencias Básicas (PEDECIBA)es
dc.description.sponsorshipConsejo Superior de Investigaciones Científicas (CSIC) I+D-Grupos 652es
dc.description.sponsorshipUniversidad de la República (UdelaR)es
dc.formatapplication/pdf
dc.format.extent1-14es
dc.identifier.issn1664-462Xes
dc.identifier.urihttp://hdl.handle.net/2133/19659
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2017.01424es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fpls.2017.01424/fulles
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderFerreira, Virginiaes
dc.rights.holderPianzzola, María J.es
dc.rights.holderVilaró, Francisco L.es
dc.rights.holderGalván, Guillermo A.es
dc.rights.holderTondo, María Lauraes
dc.rights.holderRodriguez, María V.es
dc.rights.holderOrellano, Elena G.es
dc.rights.holderValls, Marces
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectBacterial Wiltes
dc.subjectRalstonia solanacearumes
dc.subjectSolanum commersoniies
dc.subjectPotatoes
dc.subjectPlant Breedinges
dc.subjectDisease Resistancees
dc.subjectLatent Infectionses
dc.titleInterspecific potato breeding lines display differential colonization patterns and induced defense responses after Ralstonia solanacearum infectiones
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
388.pdf
Tamaño:
6.9 MB
Formato:
Adobe Portable Document Format
Descripción:
versión post-print
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.59 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Ciencias Biológicas - Artículo de Revista