KatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxide

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dc.citation.titleFrontiers in Plant Sciencees
dc.citation.volume11es
dc.creatorTondo, María Laura
dc.creatorDe Pedro-Jové, Roger
dc.creatorVandecaveye, Agustina
dc.creatorPiskulic, Laura
dc.creatorOrellano, Elena G.
dc.creatorValls, Marc
dc.date.accessioned2021-04-14T00:33:27Z
dc.date.available2021-04-14T00:33:27Z
dc.date.issued2020-07-31
dc.descriptionRalstonia solanacearum is the causative agent of bacterial wilt disease on a wide range of plant species. Besides the numerous bacterial activities required for host invasion, those involved in the adaptation to the plant environment are key for the success of infection. R. solanacearum ability to cope with the oxidative burst produced by the plant is likely one of the activities required to grow parasitically. Among the multiple reactive oxygen species (ROS)-scavenging enzymes predicted in the R. solanacearum GMI1000 genome, a single monofunctional catalase (KatE) and two KatG bifunctional catalases were identified. In this work, we show that these catalase activities are active in bacterial protein extracts and demonstrate by gene disruption and mutant complementation that the monofunctional catalase activity is encoded by katE. Different strategies were used to evaluate the role of KatE in bacterial physiology and during the infection process that causes bacterial wilt. We show that the activity of the enzyme is maximal during exponential growth in vitro and this growth-phase regulation occurs at the transcriptional level. Our studies also demonstrate that katE expression is transcriptionally activated by HrpG, a central regulator of R. solanacearum induced upon contact with the plant cells. In addition, we reveal that even though both KatE and KatG catalase activities are induced upon hydrogen peroxide treatment, KatE has a major effect on bacterial survival under oxidative stress conditions and especially in the adaptive response of R. solanacearum to this oxidant. The katE mutant strain also exhibited differences in the structural characteristics of the biofilms developed on an abiotic surface in comparison to wild-type cells, but not in the overall amount of biofilm production. The role of catalase KatE during the interaction with its host plant tomato is also studied, revealing that disruption of this gene has no effect on R. solanacearum virulence or bacterial growth in leave tissues, which suggests a minor role for this catalase in bacterial fitness in planta. Our work provides the first characterization of the R. solanacearum catalases and identifies KatE as a bona fide monofunctional catalase with an important role in bacterial protection against oxidative stress.es
dc.descriptionPara citar este articulo: Tondo ML, de Pedro-Jove´ R, Vandecaveye A, Piskulic L, Orellano EG and Valls M (2020) KatE From the Bacterial Plant Pathogen Ralstonia solanacearum Is a Monofunctional Catalase Controlled by HrpG That Plays a Major Role in Bacterial Survival to Hydrogen Peroxide. Front. Plant Sci. 11:1156. doi: 10.3389/fpls.2020.01156
dc.description.filFil: Tondo, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.es
dc.description.filFil: Tondo, María Laura. Pontificia Universidad Católica Argentina. Facultad de Química e Ingeniería del Rosario. Instituto de Ingeniería Ambiental, Química y Biotecnología Aplicada (INGEBIO -CONICET); Argentina.es
dc.description.filFil: De Pedro-Jové, Roger. Consorcio CSIC-IRTA-UAB-UB Centro de Investigación Agrogenómica (CRAG); España.es
dc.description.filFil: De Pedro-Jové, Roger. Universidad de Barcelona. Departamento de Genética; España.es
dc.description.filFil: Vandecaveye, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Piskulic, Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Estadística y Procesamiento de Datos; Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biología Molecular; Argentina.es
dc.description.filFil: Orellano, Elena G. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Valls, Marc. Consorcio CSIC-IRTA-UAB-UB Centro de Investigación Agrogenómica (CRAG); España.es
dc.description.filFil: Valls, Marc. Universidad de Barcelona. Departamento de Genética; España.es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2014-2487 y PICT 2014-2485es
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO): AGL2016-78002-R y PID2019-108595RB-I00es
dc.description.sponsorshipSevero Ochoa Program for Centers of Excellence in R&D: SEV/2015/0533es
dc.description.sponsorshipCERCA Program from the Catalan Government (Generalitat de Catalunya)es
dc.formatapplication/pdf
dc.format.extent1-12es
dc.identifier.issn1664-462Xes
dc.identifier.urihttp://hdl.handle.net/2133/20494
dc.language.isoenges
dc.publisherFrontiers Mediaes
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2020.01156es
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fpls.2020.01156/fulles
dc.rightsopenAccesses
dc.rights.holderTondo, María Lauraes
dc.rights.holderDe Pedro-Jové, Rogeres
dc.rights.holderVandecaveye, Agustinaes
dc.rights.holderPiskulic, Lauraes
dc.rights.holderOrellano, Elena G.es
dc.rights.holderValls, Marces
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectRalstonia solanacearumes
dc.subjectBacterial Wiltes
dc.subjectOxidative Burstes
dc.subjectKatE Catalasees
dc.subjectHost Adaptationes
dc.titleKatE from the bacterial plant pathogen Ralstonia solanacearum is a monofunctional catalase controlled by HrpG that plays a major role in bacterial survival to hydrogen peroxidees
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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