(FBIOyF) Departamento de Bioquímica Clínica - Artículos
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Ítem Acceso Abierto Selective inhibition of the amyloid matrix of Escherichia coli biofilms by a bifunctional microbial metabolite(Nature Research, 2023-10-19) Cordisco, Estefanía; Zanor, María Inés; Moreno, Diego M.; Serra, Diego Omar; https://orcid.org/0000-0002-6574-1702; https://orcid.org/0000-0002-8903-0673; https://orcid.org/0000-0001-5493-8537; https://orcid.org/0000-0002-3926-384XThe propensity of bacteria to grow collectively in communities known as biofilms and their ability to overcome clinical treatments in this condition has become a major medical problem, emphasizing the need for anti-biofilm strategies. Antagonistic microbial interactions have extensively served as searching platforms for antibiotics, but their potential as sources for anti-biofilm compounds has barely been exploited. By screening for microorganisms that in agar-set pairwise interactions could antagonize Escherichia coli’s ability to form macrocolony biofilms, we found that the soil bacterium Bacillus subtilis strongly inhibits the synthesis of amyloid fibers –known as curli-, which are the primary extracellular matrix (ECM) components of E. coli biofilms. We identified bacillaene, a B. subtilis hybrid non-ribosomal peptide/polyketide metabolite, previously described as a bacteriostatic antibiotic, as the effector molecule. We found that bacillaene combines both antibiotic and anti-curli functions in a concentration-dependent order that potentiates the ecological competitiveness of B. subtilis, highlighting bacillaene as a metabolite naturally optimized for microbial inhibition. Our studies revealed that bacillaene inhibits curli by directly impeding the assembly of the CsgB and CsgA curli subunits into amyloid fibers. Moreover, we found that curli inhibition occurs despite E. coli attempts to reinforce its protective ECM by inducing curli genes via a RpoS-mediated competition sensing response trigged by the threatening presence of B. subtilis. Overall, our findings illustrate the relevance of exploring microbial interactions not only for finding compounds with unknown and unique activities, but for uncovering additional functions of compounds previously categorized as antibiotics.Ítem Acceso Abierto Factors associated with mortality among hospitalized patients with COVID-19 disease treated with convalescent plasma(American Society for Microbiology, 2023-11-08) Perichon, Armando M.; Acosta, Andrea; Di Tulio, Liliana; Munuce, María José; Pezzotto, Stella; Bottasso, Oscar; http://orcid.org/0000-0002-8472-7965The use of convalescent plasma (CP) for hospitalized patients with SARSCoV-2 infection might be a useful option in certain settings. Soon after the outbreak of COVID-19, the National Ministry of Health of Argentina recommended the use of CP transfusion for hospitalized patients with COVID-19 disease. Between 1 June and 3 October 2020, 480 patients, excluding those on invasive mechanical ventilation (IMV), received at least one CP infusion in the province of Santa Fe. We aimed to find factors associated with mortality among this cohort of patients. The median age was 60 years (interquartile range: 49–69 years) and 320 (66.7%) were males. Most of these patients (93.75%) received a single CP infusion, 82.1% and 95.6% before day 4 and day 7 of hospitalization, respectively. Anti-SARS-CoV-2 titers were determined in the CP units administered using Elecsys Anti-SARS-CoV-2 S assay. At 28 days of follow-up, 250 patients were discharged (52.1%), 131 (27.3%) remained hospitalized without and 16 (3.3%) with oxygen requirement, 27 (5.6%) were on IMV, and 56 (11.7%) had died. In the multivariate logistic regression analysis, the factors significantly associated with 28-day mortality were (i) requirement of IMV, (ii) the administration of CP after the third day of hospitalization, (iii) age, and (iv) number of comorbidities. The qualitative and quantitative analyses of antibodies against SARS-CoV-2 in the infused CP were not associated with mortality. Our findings may imply a seemingly favorable effect of CP administration among patients with severe COVID-19 disease when infused sooner after hospitalization.Ítem Acceso Abierto The iron content of human serum albumin nodulates the susceptibility of Acinetobacter baumannii to Cefiderocol(MDPI, 2023-02-20) Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; Subils, Tomás; Mezcord, Vyanka; Actis, Luis A.; Tolmasky, Marcelo E.; Bonomo, Robert A.; Ramírez, María SoledadÍtem Acceso Abierto Editorial: Cell polarity: Trafficking and regulatory events that determine cell asymmetry(Frontiers Media S.A., 2023-01-12) Zucchetti, Andrés E.; Goldenring, James R.; Larocca, María CeciliaÍtem Acceso Abierto A disordered region retains the full protease inhibitor activity and the capacity to induce CD8+ T cells in vivo of the oral vaccine adjuvant U-Omp19(Elsevier, 2022-09-06) Darriba, María Laura; Pueblas Castro, Celeste; Coria, Lorena M.; Bruno, Laura; Cerutti, María Laura; Otero, Lisandro H.; Chemes, Lucía B.; Rasia, Rodolfo M.; Klinke, Sebastián; Cassataro, Juliana; Pasquevich, Karina A.Ítem Acceso Abierto Longitudinal evolution of the Pseudomonas-Derived Cephalosporinase (PDC) structure and activity in a cystic fibrosis patient treated with b-Lactams(American Society for Microbiology, 2022-09-08) Colque, Claudia A.; Albarracín Orio, Andrea G.; Tomatis, Pablo E.; Dotta, Gina; Moreno, Diego M.; Hedemann, Laura G.; Hickman, Rachel A.; Sommer, Lea M.; Feliziani, Sofía; Moyano, Alejandro J.; Bonomo, Robert A.; Johansen, Helle K.; Molin, Soren; Vila, Alejandro J.; Smania, Andrea M.Ítem Acceso Abierto Human serum albumin (HSA) regulates the expression of histone-like nucleoid structure protein (H-NS) in Acinetobacter baumannii(Nature Research, 2022-08-27) Escalante, Jenny; Nishimura, Brent; Tuttobene, Marisel Romina; Subils, Tomás; Pimentel, Camila; Georgeos, Nardin; Sieira, Rodrigo; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramírez, María SoledadÍtem Acceso Abierto Human Serum Proteins and Susceptibility of Acinetobacter baumannii to Cefiderocol: Role of Iron Transport(MDPI, 2022-03-03) Le, Casin; Pimentel, Camila; Pasteran, Fernando; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; Nishimura, Brent; Arriaga, Susana; Carranza, Aimee; Mezcord, Vyanka; Vila, Alejandro J.; Corso, Alejandra; Actis, Luis A.; Tolmasky, Marcelo E.; Bonomo, Robert A.; Ramírez, María SoledadÍtem Acceso Abierto Hyponastic leaves 1 is required for proper establishment of auxin gradient in apical hooks(American Society of Plant Biologists, 2021-10-02) Vacs, Paula; Rasia, Rodolfo M.; González Schain, NahuelÍtem Acceso Abierto Metallo-β-lactamases and a tug-of-war for the available zinc at the host–pathogen interface(Elsevier, 2022) Bahr, Guillermo; González, Lisandro J.; Vila, Alejandro J.Ítem Acceso Abierto The urgent need for metallo-β-lactamase inhibitors: an unattended global threat(Elsevier Ltd, 2021-07-08) Mojica, Maria F.; Rossi, María Agustina; Vila, Alejandro J.; Bonomo, Robert A.Ítem Acceso Abierto Deciphering the evolution of metallo-β-lactamases: A journey from the test tube to the bacterial periplasm(American Society for Biochemistry and Molecular Biology Inc., 2022-02-01) López, Carolina; Delmonti, Juliana; Bonomo, Robert A.; Vila, Alejandro J.Ítem Acceso Abierto Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the host hetermine the outcome?(MDPI, 2021-07-08) Pimentel, Camila; Le, Casin; Tuttobene, Marisel Romina; Subils, Tomás; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramírez, María SoledadÍtem Acceso Abierto Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii(Nature Research, 2021) Rodgers, Deja; Le, Casin; Pimentel, Camila; Tuttobene, Marisel Romina; Subils, Tomás; Escalante, Jenny; Nishimura, Brent; Vescovi, Eleonora García; Sieira, Rodrigo; Bonomo, Robert A.; Tolmasky, Marcelo E.; Ramírez, María SoledadÍtem Acceso Abierto Characterisation of ST25 NDM-1-producing Acinetobacter spp. strains leading the increase in NDM-1 emergence in Argentina(Elsevier, 2020-09-02) Rodgers, Deja; Pasteran, Fernando; Calderon, Manuel; Jaber, Sara; Traglia, German M.; Albornoz, Ezequiel; Corso, Alejandra; Vila, Alejandro J.; Bonomo, Robert A.; Adams, Mark D.; Ramírez, María SoledadÍtem Acceso Abierto A new model for Trypanosoma cruzi heme homeostasis depends on modulation of TcHTE protein expression(American Society for Biochemistry and Molecular Biology, 2020-07-23) Pagura, Lucas; Tevere, Evelyn; Merli, Marcelo Luciano; Cricco, Julia AlejandraÍtem Acceso Abierto Metallo-β-lactamase inhibitors inspired on snapshots from the catalytic mechanism(MDPI, 2020-06-03) Palacios, Antonela R.; Rossi, María Agustina; Mahler, Graciela S.; Vila, Alejandro J.Ítem Acceso Abierto Improved water use efficiency and shorter life cycle of Nicotiana tabacum due to modification of guard and vascular companion cells(Nature, 2018-03-12) Müller, Gabriela L.; Lara, María V.; Oitaven, Pablo; Andreo, Carlos S.; Maurino, Verónica G.; Drincovich, María FabianaÍtem Acceso Abierto Induced folding in RNA recognition by Arabidopsis thaliana DCL1(Oxford University Press, 2015-06-22) Suárez, Irina Paula; Burdisso, Paula; Benoit, Matthieu P. M. H.; Boisbouvier, Jérôme; Rasia, Rodolfo M.Ítem Acceso Abierto Efficiency and precision of microRNA biogenesis modes in plants(Oxford University Press, 2018-10-05) Moro, Belén; Chorostecki, Uciel Pablo; Arikit, Siwaret; Suárez, Irina Paula; Höbartner, Claudia; Rasia, Rodolfo M.; Meyers, Blake C.; Palatnik, Javier F.
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