Examinando por Autor "Misto, Claudia"

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  • Miniatura
    ÍtemAcceso Abierto
    Estrategias bioquímicas para el diagnóstico y monitoreo de la fibrosis quística
    (2024) Borsini, Ana; Cimino, Solange; Montenegro, Laura; Bonalumi, Paula; Di Vita, Santiago; Giordano, Joaquín; Jaccoud, Giuliana; Ledesma, Laura; Misto, Claudia; Regules, Guido; Rodríguez, María Victoria; Santos, Corina; Sterpo, Antonella; Pretto, Lautaro; Facultad de Ciencias Bioquímicas y Farmacéuticas. Carrera de Bioquímica. Práctica Profesional Obligatoria
    Introducción: la Fibrosis Quística es una enfermedad sistémica causada por la mutación de un gen localizado en el brazo largo del cromosoma 7, la cual codifica una proteína llamada Regulador de Conductancia Transmembrana de la Fibrosis Quística (CFTR). La proteína CFTR se expresa en la membrana apical de los epitelios secretores (pulmón, páncreas, intestino, glándulas sudoríparas, conductos biliares y conductos deferentes) y actúa como un transportador de cloro. Su funcionamiento defectuoso se traduce en una dificultad para transportar el cloro fuera de la célula acumulándose en el espacio intracelular. Esto genera una absorción exacerbada de sodio para mantener la electroneutralidad, por lo tanto, genera arrastre de agua. En consecuencia se produce un espesamiento de las secreciones de los epitelios comprometidos, con funcionamiento alterado de los cilios pulmonares y daño en los órganos afectados [...]
  • Miniatura
    ÍtemAcceso Abierto
    First report of canine protothecosis caused by Prototheca wickerhamii in Argentina. Brief literature review
    (Elsevier, 2025-02-15) Ramadán, Silvana; Bulacio, Lucía; Dalmaso, Hernán; Sepúlveda, Graciela; Sortino, Maximiliano Andrés; Fay, Fabián; Misto, Claudia; Salvador, María Fernanda; Etchecopaz, Alejandro; Cuestas, María Luján; https://orcid.org/0000-0002-9973-1318
    Protothecosis is an infectious disease caused by microalgae of the genus Prototheca. Prototheca can be found in soil and water and transiently colonize animals. Cutaneous protothecosis can involve not only the skin but also the underlying subcutaneous tissue and lymph nodes. This can lead to clinical signs and a microscopic tissue image that closely resembles a fungal infection. Treatment typically involves antifungal medications. We report the first case of fatal disseminated protothecosis caused by Prototheca wickerhamii in the city of Rosario, Argentina in a 5-year-old female poodle dog. The dog exhibited ocular signs of uveitis and lymphadenitis. To reach a clinical and etiological diagnosis, imaging studies, routine laboratory tests and serological tests were performed. A mycological analysis was conducted on the material obtained by puncturing three lymph nodes. Additionally, morphological, metabolic, and molecular analyses were performed. Antifungal susceptibility testing was also conducted using broth microdilution and diffusion methods. Phenotypic, metabolic, and sequencing techniques identified the isolated organism as P. wickerhamii. This isolate displayed susceptibility to amphotericin, variable susceptibility to itraconazole and voriconazole, and resistance to fluconazole and caspofungin. The frequent presence of pets in our homes highlights the need for a more comprehensive diagnostic approach. This is important because, from a public health perspective, dogs could serve as indicators of algal presence in the environments they frequently share with humans.