Induced folding in RNA recognition by Arabidopsis thaliana DCL1

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dc.citation.titleNucleic Acids Researches
dc.citation.volume43(13)es
dc.creatorSuárez, Irina Paula
dc.creatorBurdisso, Paula
dc.creatorBenoit, Matthieu P. M. H.
dc.creatorBoisbouvier, Jérôme
dc.creatorRasia, Rodolfo M.
dc.date.accessioned2021-03-04T19:59:08Z
dc.date.available2021-03-04T19:59:08Z
dc.date.issued2015-06-22
dc.descriptionDCL1 is the ribonuclease that carries out miRNA biogenesis in plants. The enzyme has two tandem double stranded RNA binding domains (dsRBDs) in its C-terminus. Here we show that the first of these domains binds precursor RNA fragments when isolated and cooperates with the second domain in the recognition of substrate RNA. Remarkably, despite showing RNA binding activity, this domain is intrinsically disordered. We found that it acquires a folded conformation when bound to its substrate, being the first report of a complete dsRBD folding upon binding. The free unfolded form shows tendency to adopt folded conformations, and goes through an unfolded bound state prior to the folding event. The significance of these results is discussed by comparison with the behavior of other dsRBDs.es
dc.descriptionThis article has been accepted for publication in Nucleic Acids Research Published by Oxford University Press.
dc.description.filFil: Suárez, Irina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Suárez, Irina Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.es
dc.description.filFil: Burdisso, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Burdisso, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.es
dc.description.filFil: Benoit, Matthieu P. M. H. Commissariat à l’énergie atomique et aux énergies alternatives (CEA). Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Benoit, Matthieu P. M. H. Centre National de la Recherche Scientifique (CNRS). Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Benoit, Matthieu P. M. H. Université Joseph Fourier. Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Boisbouvier, Jérôme. Commissariat à l’énergie atomique et aux énergies alternatives (CEA). Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Boisbouvier, Jérôme. Centre National de la Recherche Scientifique (CNRS). Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Boisbouvier, Jérôme. Université Joseph Fourier. Institut de Biologie Structurale Jean-Pierre Ebel (IBS); France.es
dc.description.filFil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR -CONICET); Argentina.es
dc.description.filFil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.es
dc.description.filThis article has been accepted for publication in Nucleic Acids Research Published by Oxford University Press.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipInternational cooperation grant res. 962/2009es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT-2007-720, PICT-2008-318 y PICT-2012-1702 (founding for open access charge)es
dc.formatapplication/pdf
dc.format.extent6607–6619es
dc.identifier.issn1362-4962es
dc.identifier.urihttp://hdl.handle.net/2133/20017
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkv627es
dc.relation.publisherversionhttps://academic.oup.com/nar/article/43/13/6607/2414337es
dc.rightsopenAccesses
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.holderSuárez, Irina Paulaes
dc.rights.holderBurdisso, Paulaes
dc.rights.holderBenoit, Matthieu P. M. H.es
dc.rights.holderBoisbouvier, Jérômees
dc.rights.holderRasia, Rodolfo M.es
dc.rights.textAttribution 4.0 International (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectDcl1es
dc.subjectMicroRNAses
dc.subjectDouble-stranded RNA-Binding Domain (dsRBD)es
dc.subjectIntrinsically Disordered Proteinses
dc.titleInduced folding in RNA recognition by Arabidopsis thaliana DCL1es
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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