An innovative bio-vehicle for resveratrol and tocopherol based on quinoa 11S globulin-nanocomplex design and characterization

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dc.citation.titlePharmaceutics
dc.citation.volume16
dc.contributor.orcidhttps://orcid.org/0009-0006-4857-4769
dc.contributor.orcidhttps://orcid.org/0000-0002-8223-1077
dc.creatorRubinstein, Alejandra J.
dc.creatorGarcía Liñares, Guadalupe
dc.creatorBoeris, Valeria
dc.creatorPérez, Oscar E.
dc.date.accessioned2025-03-06T10:58:27Z
dc.date.available2025-03-06T10:58:27Z
dc.date.issued2024-08-24
dc.description.abstractNanocomplexes, which possess immense potential to function as nanovehicles, can link diverse ligand compounds. The objective of the present study was to design and characterize resveratrol (RSV)- and tocopherol (TOC)-loaded 11S quinoa seed protein nanocomplexes. Firstly, molecular docking was performed to describe the probable binding sites between protein and ligands, and binding energies of −5.6 and −6.2 kcal/mol were found for RSV and TOC, respectively. Isothermal titration calorimetry allowed us to obtain the thermodynamic parameters that described the molecular interactions between RSV or TOC with the protein, finding the complexation process to be exothermic and spontaneous. 11S globulin intrinsic fluorescence spectra showed quenching effects exerted by RSV and TOC, demonstrating protein–bioactive compound interactions. The application of Stern–Volmer, Scatchard, and Förster resonance energy transfer models confirmed static quenching and allowed us to obtain parameters that described the 11S-RSV and 11S-TOC complexation processes. RSV has a higher tendency to bind 11S globulin according to ITC and fluorescence analysis. Secondly, the protein aggregation induced by bioactive compound interactions was confirmed by dynamic light scattering and atomic force microscopy, with diameters <150 nm detected by both techniques. Finally, it was found that the antioxidant capacity of a single 11S globulin did not decrease; meanwhile, it was additive for 11S-RSV. These nanocomplexes could constitute a real platform for the design of nutraceutical products.
dc.description.filFil: Rubinstein, Alejandra J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina.
dc.description.filFil: Rubinstein, Alejandra J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica (IQUIBICEN-CONICET); Argentina.
dc.description.filFil: García Liñares, Guadalupe. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Laboratorio de Biocatálisis; Argentina.
dc.description.filFil: García Liñares, Guadalupe. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Unidad de Microanálisis y Métodos Físicos en Química Orgánica (UMYMFOR-CONICET); Argentina.
dc.description.filFil: Boeris, Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Física. Área Fisicoquímica; Argentina.
dc.description.filFil: Boeris, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina.
dc.description.filFil: Pérez, Oscar E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina.
dc.description.filFil: Pérez, Oscar E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica (IQUIBICEN-CONICET); Argentina.
dc.description.sponsorshipUniversidad de Buenos Aires: UBACYT Nº 20020190100297BA
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT 2021-0347
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP 2022-2024 GI 11220210100072CO
dc.description.versionpeerreviewed
dc.format.extent1-22
dc.identifier.e-issn1999-4923
dc.identifier.urihttps://hdl.handle.net/2133/28994
dc.language.isoen
dc.publisherMDPI
dc.relation.publisherversionhttps://doi.org/10.3390/pharmaceutics16091118
dc.relation.publisherversionhttps://www.mdpi.com/1999-4923/16/9/1118
dc.rightsopenAccess
dc.rights.holderRubinstein, Alejandra J.
dc.rights.holderGarcía Liñares, Guadalupe
dc.rights.holderBoeris, Valeria
dc.rights.holderPérez, Oscar E.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject11S globulin
dc.subjectQuinoa
dc.subjectBioactive compounds
dc.subjectComplexation
dc.subjectAggregation
dc.subjectNutraceutical products
dc.titleAn innovative bio-vehicle for resveratrol and tocopherol based on quinoa 11S globulin-nanocomplex design and characterization
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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