The Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiae

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dc.citation.titleFEMS Microbiology Letterses
dc.citation.volume312(2)es
dc.creatorBuchensky, Celeste
dc.creatorAlmirón, Paula
dc.creatorSuarez Mantilla, Brian
dc.creatorSilber, Ariel M.
dc.creatorCricco, Julia Alejandra
dc.date.accessioned2020-06-08T15:01:51Z
dc.date.available2020-06-08T15:01:51Z
dc.date.issued2010-11
dc.descriptionTrypanosoma cruzi, the etiologic agent for Chagas' disease, has requirements for several cofactors, one of which is heme. Because this organism is unable to synthesize heme, which serves as a prosthetic group for several heme proteins (including the respiratory chain complexes), it therefore must be acquired from the environment. Considering this deficiency, it is an open question as to how heme A, the essential cofactor for eukaryotic CcO enzymes, is acquired by this parasite. In the present work, we provide evidence for the presence and functionality of genes coding for heme O and heme A synthases, which catalyze the synthesis of heme O and its conversion into heme A, respectively. The functions of these T. cruzi proteins were evaluated using yeast complementation assays, and the mRNA levels of their respective genes were analyzed at the different T. cruzi life stages. It was observed that the amount of mRNA coding for these proteins changes during the parasite life cycle, suggesting that this variation could reflect different respiratory requirements in the different parasite life stages.es
dc.description.filFil: Buchensky, Celeste. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Almirón, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.filFil: Suarez Mantilla, Brian. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia; Brazil.es
dc.description.filFil: Silber, Ariel M. Universidade de São Paulo. Instituto de Ciências Biomédicas. Departamento de Parasitologia; Brazil.es
dc.description.filFil: Cricco, Julia Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química Biológica. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)es
dc.description.sponsorshipFundacão de Amparo à Pesquisa do Estado de São Paulo (FAPESP, project #08-57596-4)es
dc.description.sponsorshipCNPq (Project #473906/2008-2)es
dc.description.sponsorshipInstituto Nacional de Biotecnologia Estrutural e Química Medicinal em Doenças Infecciosas, INBEQMeDI (Brazil)es
dc.formatapplication/pdf
dc.format.extent133–141es
dc.identifier.issn0378-1097es
dc.identifier.urihttp://hdl.handle.net/2133/18322
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.publisherversionhttps://doi.org/10.1111/j.1574-6968.2010.02109.xes
dc.relation.publisherversionhttps://academic.oup.com/femsle/article/312/2/133/529316es
dc.rightsopenAccesses
dc.rights.holderBuchensky, Celestees
dc.rights.holderAlmirón, Paulaes
dc.rights.holderSuarez Mantilla, Brianes
dc.rights.holderSilber, Ariel M.es
dc.rights.holderCricco, Julia Alejandraes
dc.rights.holderFederation of European Microbiological Societieses
dc.rights.holderOxford University Presses
dc.rights.holderWiley-Blackwelles
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTrypanosoma cruzies
dc.subjectheme Aes
dc.subjectcytochrome c oxidasees
dc.subjectmitochondrial proteinses
dc.subjectChagas' diseasees
dc.titleThe Trypanosoma cruzi proteins TcCox10 and TcCox15 catalyze the formation of heme A in the yeast Saccharomyces cerevisiaees
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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