Water extract from inflorescences of industrial hemp Futura 75 variety as a source of anti-inflammatory, anti-proliferative and antimycotic agents: results from in silico, in vitro and ex vivo studies

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dc.citation.titleAntioxidants
dc.citation.volume9
dc.creatorOrlando, Giustino
dc.creatorRecinella, Lucia
dc.creatorChiavaroli, Annalisa
dc.creatorBrunetti, Luigi
dc.creatorLeone, Sheila
dc.creatorCarradori, Simone
dc.creatorDi Simone, Simonetta
dc.creatorCiferri, Maria Chiara
dc.creatorZengin, Gokhan
dc.creatorAk, Gunes
dc.creatorAbdullah, Hassan H.
dc.creatorCordisco, Estefanía
dc.creatorSortino, Maximiliano Andrés
dc.creatorSvetaz, Laura Andrea
dc.creatorPoliti, Matteo
dc.creatorAngelini, Paola
dc.creatorCovino, Stefano
dc.creatorVenanzoni, Roberto
dc.creatorCesa, Stefania
dc.creatorMenghini, Luigi
dc.creatorFerrante, Claudio
dc.date.accessioned2025-02-05T12:31:31Z
dc.date.available2025-02-05T12:31:31Z
dc.date.issued2020-05-17
dc.description.abstractIndustrial hemp (Cannabis sativa) is traditionally cultivated as a valuable source of fibers and nutrients. Multiple studies also demonstrated antimicrobial, anti-proliferative, phytotoxic and insecticide effects of the essential oil from hemp female inflorescences. On the other side, only a few studies explored the potential pharmacological application of polar extracts from inflorescences. In the present study, we investigated the water extract from inflorescences of industrial hemp Futura 75 variety, from phytochemical and pharmacological point of view. The water extract was assayed for phenolic compound content, radical scavenger/reducing, chelating and anti-tyrosinase effects. Through an ex vivo model of toxicity induced by lipopolysaccharide (LPS) on isolated rat colon and liver, we explored the extract effects on serotonin, dopamine and kynurenine pathways and the production of prostaglandin (PG)E2. Anti-proliferative effects were also evaluated against human colon cancer HCT116 cell line. Additionally, antimycotic effects were investigated against Trichophyton rubrum, Trichophyton interdigitale, Microsporum gypseum. Finally, in silico studies, including bioinformatics, network pharmacology and docking approaches were conducted in order to predict the putative targets underlying the observed pharmacological and microbiological effects. Futura 75 water extract was able to blunt LPS-induced reduction of serotonin and increase of dopamine and kynurenine turnover, in rat colon. Additionally, the reduction of PGE2 levels was observed in both colon and liver specimens, as well. The extract inhibited the HCT116 cell viability, the growth of T. rubrum and T. interdigitale and the activity of tyrosinase, in vitro, whereas in silico studies highlighting the inhibitions of cyclooxygenase-1 (induced by carvacrol), carbonic anhydrase IX (induced by chlorogenic acid and gallic acid) and lanosterol 14-α-demethylase (induced by rutin) further support the observed pharmacological and antimycotic effects. The present findings suggest female inflorescences from industrial hemp as high quality by-products, thus representing promising sources of nutraceuticals and cosmeceuticals against inflammatory and infectious diseases.
dc.description.filFil: Orlando, Giustino. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Recinella, Lucia. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Chiavaroli, Annalisa. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Brunetti, Luigi. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Leone, Sheila. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Carradori, Simone. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Di Simone, Simonetta. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Ciferri, Maria Chiara. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Zengin, Gokhan. Selcuk Universtiy. Science Faculty. Department of Biology; Turkey.
dc.description.filFil: Ak, Gunes. Selcuk Universtiy. Science Faculty. Department of Biology; Turkey.
dc.description.filFil: Abdullah, Hassan H. Salahaddin University-Erbil. College of Education. Chemistry Department; Iraq.
dc.description.filFil: Abdullah, Hassan H. Universiti Sains Malaysia. School of Pharmaceutical Sciences; Malaysia.
dc.description.filFil: Cordisco, Estefanía. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacognosia; Argentina.
dc.description.filFil: Sortino, Maximiliano Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacognosia; Argentina.
dc.description.filFil: Sortino, Maximiliano Andrés. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina.
dc.description.filFil: Svetaz, Laura Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Farmacognosia; Argentina.
dc.description.filFil: Politi, Matteo. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Angelini, Paola. Università degli Studi di Perugia. Dipartimento di Chimica, biologia e biotecnologie; Italia.
dc.description.filFil: Covino, Stefano. Università degli Studi di Perugia. Dipartimento di Chimica, biologia e biotecnologie; Italia.
dc.description.filFil: Venanzoni, Roberto. Università degli Studi di Perugia. Dipartimento di Chimica, biologia e biotecnologie; Italia.
dc.description.filFil: Cesa, Stefania. Sapienza Università di Roma. Dipartimento di Chimica e Tecnologie del Farmaco; Italia.
dc.description.filFil: Menghini, Luigi. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.filFil: Ferrante, Claudio. Università degli Studi "G. d'Annunzio". Dipartimento di Farmacia; Italia.
dc.description.sponsorshipItalian Ministry for Universities and Research (MUR): FAR 2019, FAR 2014
dc.description.versionpeerreviewed
dc.format.extent1-19
dc.identifier.e-issn2076-3921
dc.identifier.urihttps://hdl.handle.net/2133/28791
dc.language.isoen
dc.publisherMDPI
dc.relation.publisherversionhttps://doi.org/10.3390/antiox9050437
dc.relation.publisherversionhttps://www.mdpi.com/2076-3921/9/5/437
dc.rightsopenAccess
dc.rights.holderOrlando, Giustino
dc.rights.holderRecinella, Lucia
dc.rights.holderChiavaroli, Annalisa
dc.rights.holderBrunetti, Luigi
dc.rights.holderLeone, Sheila
dc.rights.holderCarradori, Simone
dc.rights.holderDi Simone, Simonetta
dc.rights.holderCiferri, Maria Chiara
dc.rights.holderZengin, Gokhan
dc.rights.holderAk, Gunes
dc.rights.holderAbdullah, Hassan H.
dc.rights.holderCordisco, Estefanía
dc.rights.holderSortino, Maximiliano Andrés
dc.rights.holderSvetaz, Laura Andrea
dc.rights.holderPoliti, Matteo
dc.rights.holderAngelini, Paola
dc.rights.holderCovino, Stefano
dc.rights.holderVenanzoni, Roberto
dc.rights.holderCesa, Stefania
dc.rights.holderMenghini, Luigi
dc.rights.holderFerrante, Claudio
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectFutura 75
dc.subjectSerotonin
dc.subjectDopamine
dc.subjectKynurenine
dc.subjectAntimycotic
dc.subjectAnti-proliferative
dc.subjectAnti-inflammatory
dc.subjectAntimycotic agents
dc.subjectCannabis sativa
dc.titleWater extract from inflorescences of industrial hemp Futura 75 variety as a source of anti-inflammatory, anti-proliferative and antimycotic agents: results from in silico, in vitro and ex vivo studies
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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