Anotación genómica y análisis comparativo entre bacteriófagos de Staphylococcus aureus
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Staphylococcus aureus es un patógeno bacteriano capaz de causar una amplia
variedad de enfermedades tanto en humanos como en animales. Con el fin de
entender la mecánica de su patogenia y de buscar tratamientos alternativos es que
se recurre al uso de bacteriófagos. Los mismos son virus que infectan bacterias de
manera altamente específica y tienen la capacidad de destruirlas. En este trabajo se
realizó la anotación y el análisis comparativo de cuatro bacteriófagos provenientes
de diferentes orígenes capaces de infectar S. aureus, dos de ellos líticos y dos
temperados. Centrándose en sus diferencias se propuso la utilización de diferentes
herramientas bioinformáticas y el análisis de diferentes genes para su estudio. Se
logró caracterizar a los bacteriófagos y analizar tanto las diferencias como las
similitudes genéticas con los pertenecientes a su misma familia. Se analizaron las
endolisinas codificadas por estos fagos, de manera interesante un fago
(vB_SauS_308) solo contiene el dominio CHAP de ésta. Por último, se realizó la
búsqueda de genes que codifiquen factores de virulencia y toxinas.
Staphylococcus aureus is a bacterial pathogen capable of causing a variety of diseases both in humans and in animals. We resort to the use of bacteriophages in order to understand the pathogenesis and look for alternative treatments. Phages are viruses that infect bacteria in a specific way and have the ability to lyse them. In this study the annotation and the comparative analysis of four bacteriophages from environmental, animal and human origin capable of infect S. aureus was carried out. Considering the differences between the phages the utilization of different bioinformatics tools and the selection of distinct genes has been proposed. It has been possible to describe the distinctive features and the similarities with those belonging to the same family. Endolysins were analysed, and one phage (vB_SauS_308) only shows CHAP domain. To finalize the search for genes encoding virulence factors and toxins was carried out.
Staphylococcus aureus is a bacterial pathogen capable of causing a variety of diseases both in humans and in animals. We resort to the use of bacteriophages in order to understand the pathogenesis and look for alternative treatments. Phages are viruses that infect bacteria in a specific way and have the ability to lyse them. In this study the annotation and the comparative analysis of four bacteriophages from environmental, animal and human origin capable of infect S. aureus was carried out. Considering the differences between the phages the utilization of different bioinformatics tools and the selection of distinct genes has been proposed. It has been possible to describe the distinctive features and the similarities with those belonging to the same family. Endolysins were analysed, and one phage (vB_SauS_308) only shows CHAP domain. To finalize the search for genes encoding virulence factors and toxins was carried out.