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Structural determinants of Arabidopsis thaliana Hyponastic Leaves 1 function in vivo

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dc.citation.titlePLoS ONE
dc.citation.volume9
dc.creatorBurdisso, Paula
dc.creatorMilia, Fernando
dc.creatorSchapire, Arnaldo L.
dc.creatorBologna, Nicolás G.
dc.creatorPalatnik, Javier F.
dc.creatorRasia, Rodolfo M.
dc.date.accessioned2024-06-07T16:10:04Z
dc.date.available2024-06-07T16:10:04Z
dc.date.issued2014-11-19
dc.description.abstractMicroRNAs have turned out to be important regulators of gene expression. These molecules originate from longer transcripts that are processed by ribonuclease III (RNAse III) enzymes. Dicer proteins are essential RNAse III enzymes that are involved in the generation of microRNAs (miRNAs) and other small RNAs. The correct function of Dicer relies on the participation of accessory dsRNA binding proteins, the exact function of which is not well-understood so far. In plants, the double stranded RNA binding protein Hyponastic Leaves 1 (HYL1) helps Dicer Like protein (DCL1) to achieve an efficient and precise excision of the miRNAs from their primary precursors. Here we dissected the regions of HYL1 that are essential for its function in Arabidopsis thaliana plant model. We generated mutant forms of the protein that retain their structure but affect its RNA-binding properties. The mutant versions of HYL1 were studied both in vitro and in vivo, and we were able to identify essential aminoacids/residues for its activity. Remarkably, mutation and even ablation of one of the purportedly main RNA binding determinants does not give rise to any major disturbances in the function of the protein. We studied the function of the mutant forms in vivo, establishing a direct correlation between affinity for the pri-miRNA precursors and protein activity.
dc.description.filFil: Burdisso, Paula. Instituto de Biología Molecular y Celular de Rosario; Argentina.
dc.description.filFil: Burdisso, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.
dc.description.filFil: Milia, Fernando. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.
dc.description.filFil: Schapire, Arnaldo L. Instituto de Biología Molecular y Celular de Rosario; Argentina.
dc.description.filFil: Schapire, Arnaldo L. Center for Research in Agricultural Genomics (CRAG); Spain.
dc.description.filFil: Bologna, Nicolás G. Instituto de Biología Molecular y Celular de Rosario; Argentina.
dc.description.filFil: Bologna, Nicolás G. Swiss Federal Institute of Technology (ETH); Switzerland.
dc.description.filFil: Palatnik, Javier F. Instituto de Biología Molecular y Celular de Rosario; Argentina.
dc.description.filFil: Rasia, Rodolfo M. Instituto de Biología Molecular y Celular de Rosario; Argentina.
dc.description.filFil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCyT): PICT 2007-720, 2008-318
dc.description.versionpeerreviewed
dc.format.extent1-8
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/2133/27279
dc.language.isoen
dc.publisherPublic Library of Science
dc.relation.publisherversionhttps://doi.org/10.1371/journal.pone.0113243
dc.relation.publisherversionhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113243
dc.rightsopenAccess
dc.rights.holderBurdisso, Paula
dc.rights.holderMilia, Fernando
dc.rights.holderSchapire, Arnaldo L.
dc.rights.holderBologna, Nicolás G.
dc.rights.holderPalatnik, Javier F.
dc.rights.holderRasia, Rodolfo M.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectArabidopsis proteins
dc.subjectCell cycle
dc.subjectGene expression regulation
dc.subjectProtein structure
dc.subjectRibonuclease III
dc.titleStructural determinants of Arabidopsis thaliana Hyponastic Leaves 1 function in vivo
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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