Interaction of Acinetobacter baumannii with Human Serum Albumin: Does the host hetermine the outcome?

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dc.citation.titleAntibioticses
dc.citation.volume10es
dc.creatorPimentel, Camila
dc.creatorLe, Casin
dc.creatorTuttobene, Marisel Romina
dc.creatorSubils, Tomás
dc.creatorBonomo, Robert A.
dc.creatorTolmasky, Marcelo E.
dc.creatorRamírez, María Soledad
dc.date.accessioned2022-03-29T21:46:42Z
dc.date.available2022-03-29T21:46:42Z
dc.date.issued2021-07-08
dc.descriptionAcinetobacter baumannii has become a serious threat to human health due to its extreme antibiotic resistance, environmental persistence, and capacity to survive within the host. Two A. baumannii strains, A118 and AB5075, commonly used as model systems, and three carbapenemresistant strains, which are becoming ever more dangerous due to the multiple drugs they can resist, were exposed to 3.5% human serum albumin (HSA) and human serum (HS) to evaluate their responsewithrespecttoantimicrobialresistance,biofilmformation,andquorumsensing,allfeatures responsible for increasing survival and persistence in the environment and human body. Expression levels of antibiotic resistance genes were modified differently when examined in different strains. The cmlA gene was upregulated or downregulated in conditions of exposure to 3.5% HSA or HS dependingonthestrain. Expressionlevelsofpbp1andpbp3tendedtobeincreasedbythepresenceof HSA and HS, but the effect was not seen in all strains. A. baumannii A118 growing in the presence of HS did not experience increased expression of these genes. Aminoglycoside-modifying enzymes were also expressed at higher or lower levels in the presence of HSA or HS. Still, the response was not uniform; in some cases, expression was enhanced, and in other cases, it was tapered. While A. baumannii AB5075 became more susceptible to rifampicin in the presence of 3.5% HSA or HS, strain A118 did not show any changes. Expression of arr2, a gene involved in resistance to rifampicin present in A.baumannii AMA16, was expressed at higher levels when HS was present in the culture medium. HSA and HS reduced biofilm formation and production of N-Acyl Homoserine Lactone, a compound intimately associated with quorum sensing. In conclusion, HSA, the main component of HS, stimulates a variety of adaptative responses in infecting A. baumannii strains.es
dc.description.filFil: Papp-Wallace, Krisztina M. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics. Cleveland.es
dc.description.filFil: Papp-Wallace, Krisztina M. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES)es
dc.description.filFil: Bonomo, Robert A. Louis Stokes Cleveland Department of Veterans Affairs Medical Center. Research Service and GRECC. Cleveland.es
dc.description.filFil: Bonomo, Robert A. Case Western Reserve University School of Medicine. Departments of Medicine, Pharmacology, Molecular Biology and Microbiology, Biochemistry, Proteomics and Bioinformatics. Cleveland.es
dc.description.filFil: Bonomo, Robert A. CWRU-Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES)es
dc.description.filFil: Tomasky, Marcelo E. College of Natural Sciences and Mathematics. Department of Biological Science. Fullerton.es
dc.description.sponsorshipCleveland Department of Veterans Affairs: 1I01 BX002872, 1I01BX001974
dc.description.sponsorshipNational Institutes of Health (NIH): 2R15 AI047115, MHRT 2T37MD001368, R01AI063517, R01AI072219, R01AI100560, SC3GM125556
dc.description.sponsorshipNational Institute on Minority Health and Health Disparities (NIMHD)
dc.description.sponsorshipBiomedical Laboratory Research and Development, VA Office of Research and Development
dc.description.sponsorshipGeriatric Research Education and Clinical Center (GRECC)
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.formatapplication/pdf
dc.format.extent1-14es
dc.identifier.issn2079-6382es
dc.identifier.urihttp://hdl.handle.net/2133/23288
dc.language.isoenges
dc.publisherMDPIes
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/10/7/833es
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics10070833
dc.rightsopenAccesses
dc.rights.holderPimentel, Camilaes
dc.rights.holderLe, Casines
dc.rights.holderTuttobene, Marisel Rominaes
dc.rights.holderSubils, Tomáses
dc.rights.holderPapp-Wallace, Krisztina M.es
dc.rights.holderBonomo, Robert A.es
dc.rights.holderTolmasky, Marcelo E.es
dc.rights.holderRamírez, María Soledades
dc.rights.textAtribución 4.0 Internacional (CC BY 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/deed.es*
dc.subjectAcinetobacter Baumanniies
dc.subjectAntibiotic Resistancees
dc.subjectQuorum Sensinges
dc.subjectBiofilmes
dc.subjectHuman Serumes
dc.subjectAlbumines
dc.titleInteraction of Acinetobacter baumannii with Human Serum Albumin: Does the host hetermine the outcome?es
dc.typepublishedVersion
dc.typearticle
dc.typeartículo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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