Expression and purification of untagged GlnK proteins from actinobacteria

Vista sencilla de ítem
dc.citation.titleEXCLI Journal
dc.citation.volume949-958
dc.citation.volume16
dc.creatorGerhardt, Edileusa C. M.
dc.creatorMoure, Vivian R.
dc.creatorSouza, Andrey W.
dc.creatorPedrosa, Fabio O.
dc.creatorSouza, Emanuel M.
dc.creatorDiacovich, Lautaro
dc.creatorGramajo, Hugo Cesar
dc.creatorHuergo, Luciano F.
dc.date.accessioned2024-04-19T19:03:54Z
dc.date.available2024-04-19T19:03:54Z
dc.date.issued2017-06-27
dc.description.abstractThe PII protein family constitutes one of the most conserved and well distributed family of signal transduction proteins in nature. These proteins play key roles in nitrogen and carbon metabolism. PII function has been well documented in Gram-negative bacteria. However, there are very few reports describing the in vitro properties and function of PII derived from Gram-positive bacteria. Here we present the heterologous expression and efficient purification protocols for untagged PII from three Actinobacteria of medical and biotechnological interest namely: Mycobacterium tuberculosis, Rhodococcus jostii and Streptomyces coelicolor. Circular dichroism and gel filtration analysis supported that the purified proteins are correctly folded. The purification protocol described here will facilitate biochemical studies and help to uncover the biochemical functions of PII proteins in Actinobacteria.
dc.description.filFil: Gerhardt, Edileusa C.M. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Moure, Vivian R. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Souza, Andrey W. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Pedrosa, Fabio O. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Souza, Emanuel M. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Diacovich, Lautaro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmaceuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gramajo, Hugo Cesar. Universidad Nacional de Rosario. Facultad de Ciencias Bioquimicas y Farmaceuticas. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Huergo, Luciano F. Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular; Brazil.
dc.description.filFil: Huergo, Luciano F. Universidade Federal do Paraná. Setor Litoral; Brazil.
dc.description.sponsorshipFundação Araucária
dc.description.sponsorshipInstitutos Nacionais de Ciência, Tecnologia e Inovação (INCT)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.identifier.issn1611-2156
dc.identifier.urihttps://hdl.handle.net/2133/26912
dc.language.isoen
dc.publisherEXCLI
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5579400/
dc.relation.publisherversionhttps://doi.org/10.17179%2Fexcli2017-394
dc.rightsopenAccess
dc.rights.holderGerhardt, Edileusa C.M.
dc.rights.holderMoure, Vivian R.
dc.rights.holderSouza, Andrey W.
dc.rights.holderPedrosa, Fabio O.
dc.rights.holderSouza, Emanuel M.
dc.rights.holderDiacovich, Lautaro
dc.rights.holderGramajo, Hugo Cesar
dc.rights.holderHuergo, Luciano F.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectPII
dc.subjectGlnK
dc.subjectCD analysis
dc.subjectActinobacteria
dc.titleExpression and purification of untagged GlnK proteins from actinobacteria
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

Archivos

Bloque original
Mostrando 1 - 1 de 1
Miniatura
Nombre:
Expression and purification of untagged GlnK proteins from actinobacteria.pdf
Tamaño:
602.19 KB
Formato:
Adobe Portable Document Format
Descargar
Bloque de licencias
Mostrando 1 - 1 de 1
Nombre:
license.txt
Tamaño:
3.87 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Descargar

Colecciones

(FBIOyF) Departamento de Química Orgánica - Artículos