Simultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”

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dc.citation.titleCell Biochemistry and Biophysicses
dc.creatorLondero, Carolina M.
dc.creatorRiquelme, Bibiana Doris
dc.date.accessioned2020-12-02T17:38:32Z
dc.date.available2020-12-02T17:38:32Z
dc.date.issued2020-11-07
dc.descriptionThe simultaneous determination of adhesion and deformability parameters of erythrocytes was carried out through a microfluidic device, which uses an inverted optical microscope with new image acquisition and analysis technologies. Also, an update of the models describing erythrocyte adhesion and deformation was proposed. Measurements were carried out with red blood cells suspended in saline solution with human serum albumin at different concentrations. Erythrocytes adhered to a glass surface were subjected to different low shear stress (from 0.04 to 0.25 Pa), causing cellular deformation and dissociation. The maximum value obtained of the erythrocyte deformability index was 0.3, and that of the adhesion energy per unit area was 1.1 × 10-6 Pa m, both according to previous works. The obtained images of RBCs adhered to glass reveal that the adhesion is stronger in a single point of the cell, suggesting a ligand migration that concentrates the adhesion in a "spike-like tip" in the cell. Moreover, adhesion energy results indicate that the energy required to separate erythrocytes in media with a lower albumin concentration is greater. Both results could be explained by the mobility of membrane receptors.es
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Cell Biochemistry and Biophysics. The final authenticated version is available online at: https://doi.org/10.1007/s12013-020-00956-9en
dc.description.filFil: Londero, Carolina M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.filFil: Londero, Carolina M. Universidad Nacional de Rosario. Facultad de Ciencias Exactas, Ingeniería y Agrimensura; Argentina.es
dc.description.filFil: Riquelme, Bibiana Doris. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Física Rosario (IFIR-CONICET); Argentina.es
dc.description.sponsorshipUniversidad Nacional de Rosarioes
dc.formatapplication/pdf
dc.format.extent0es
dc.identifier.issn1559-0283es
dc.identifier.urihttp://hdl.handle.net/2133/19394
dc.language.isoenges
dc.publisherSpringeres
dc.relation.publisherversionhttps://doi.org/10.1007/s12013-020-00956-9es
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s12013-020-00956-9#Abs1es
dc.rightsembargoedAccesses
dc.rights.holderSpringeres
dc.rights.holderLondero, Carolina M.es
dc.rights.holderRiquelme, Bibiana Dorises
dc.rights.holderUniversidad Nacional de Rosarioes
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)es
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectHemorheologyes
dc.subjectMicrofluidic chamberes
dc.subjectRed blood cell adhesiones
dc.subjectRed blood cell deformationes
dc.titleSimultaneous determination of Human Erythrocyte Deformability and Adhesion Energy: a novel approach using a microfluidic chamber and the “Glass Effect”es
dc.typearticle
dc.typeartículo
dc.typepublishedVersion
dc.type.collectionarticulo
dc.type.versionpublishedVersiones

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