Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity

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dc.citation.titleThe Plant Journal
dc.citation.volume79
dc.contributor.otherWang, Jia-Wei: provide the vectors containing the AS1 and ANT promoter
dc.contributor.otherWeigel, Detlef: provide the vectors containing the AS1 and ANT promoter
dc.creatorDebernardi, Juan Manuel
dc.creatorMecchia, Martín Alejandro
dc.creatorVercruyssen, Liesbeth
dc.creatorSmaczniak, Cezary
dc.creatorKaufmann, Kerstin
dc.creatorInze, Dirk
dc.creatorRodríguez, Ramiro Esteban
dc.creatorPalatnik, Javier F.
dc.date.accessioned2024-11-19T11:19:18Z
dc.date.available2024-11-19T11:19:18Z
dc.date.issued2014-06-02
dc.description.abstractThe growth-regulating factors (GRFs) are plant-specific transcription factors. They form complexes with GRF-interacting factors (GIFs), a small family of transcriptional co-activators. In Arabidopsis thaliana, seven out of the nine GRFs are controlled by microRNA miR396. Analysis of Arabidopsis plants carrying a GRF3 allele insensitive to miR396 revealed a strong boost in the number of cells in leaves, which was further enhanced synergistically by an additional increase of GIF1 levels. Genetic experiments revealed that GRF3 can still increase cell number in gif1 mutants, albeit to a much lesser extent. Genome-wide transcript profiling indicated that the simultaneous increase of GRF3 and GIF1 levels causes additional effects in gene expression compared to either of the transgenes alone. We observed that GIF1 interacts in vivo with GRF3, as well as with chromatin-remodeling complexes, providing a mechanistic explanation for the synergistic activities of a GRF3–GIF1 complex. Interestingly, we found that, in addition to the leaf size, the GRF system also affects the organ longevity. Genetic and molecular analysis revealed that the functions of GRFs in leaf growth and senescence can be uncoupled, demonstrating that the miR396-GRF-GIF network impinges on different stages of leaf development. Our results integrate the post-transcriptional control of the GRF transcription factors with the progression of leaf development.
dc.description.filFil: Debernardi, Juan Manuel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Mecchia, Martín Alejandro. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Vercruyssen, Liesbeth. Department of Plant Systems Biology; Belgium.
dc.description.filFil: Smaczniak, Cezary. Wageningen University & Research. Laboratory of Molecular Biology; Netherlands.
dc.description.filFil: Kaufmann, Kerstin. Wageningen University & Research. Laboratory of Molecular Biology; Netherlands.
dc.description.filFil: Inze, Dirk. Department of Plant Systems Biology; Belgium.
dc.description.filFil: Rodríguez, Ramiro Esteban. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Palatnik, Javier F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
dc.description.sponsorshipEuropean Molecular Biology Organization (EMBO)
dc.description.sponsorshipFundacion Josefina Prats
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i)
dc.description.sponsorshipNetherlands Organisation for Scientific Research (NWO): VIDI grant
dc.description.sponsorshipNetherlands Proteomics Centre (NPC)
dc.description.sponsorshipCentre for BioSystems Genomics (CBSG)
dc.description.sponsorshipGhent University. "Bijzonder Onderzoeksfonds Methusalem" Project: BOF08/01M00408
dc.description.sponsorshipGhent University. Multidisciplinary Research Partnership "Biotechnology for a Sustainable Economy" Project: 01MRB510W
dc.description.sponsorshipBelgian Science Policy Office (BELSPO): IUAP VI/33
dc.description.sponsorshipEuropean Union FP6. "AGRON-OMICS": Grant LSHG-CT-2006-037704
dc.description.versionpeerreviewed
dc.format.extent1-14
dc.identifier.e-issn1365-313X
dc.identifier.issn0960-7412
dc.identifier.urihttps://hdl.handle.net/2133/28156
dc.language.isoen
dc.publisherWiley
dc.relation.publisherversionhttps://doi.org/10.1111/tpj.12567
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/10.1111/tpj.12567
dc.rightsopenAccess
dc.rights.holderDebernardi, Juan Manuel
dc.rights.holderMecchia, Martín Alejandro
dc.rights.holderVercruyssen, Liesbeth
dc.rights.holderSmaczniak, Cezary
dc.rights.holderKaufmann, Kerstin
dc.rights.holderInze, Dirk
dc.rights.holderRodríguez, Ramiro Esteban
dc.rights.holderPalatnik, Javier F.
dc.rights.holderUniversidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectmiR396
dc.subjectGrowth-regulating factor
dc.subjectGRF-interacting factors
dc.subjectAN3
dc.subjectProliferation
dc.subjectSenescence
dc.titlePost-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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