The type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation

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dc.citation.titleBMC Microbiology
dc.citation.volume14
dc.creatorZimaro, Tamara
dc.creatorSgro, Germán Gustavo
dc.creatorGarofalo, Cecilia Graciela
dc.creatorFicarra, Florencia Andrea
dc.creatorOttado, Jorgelina
dc.creatorGottig, Natalia
dc.creatorThomas, Ludivine
dc.creatorMarondedze, Claudius
dc.creatorGehring, Chris
dc.date.accessioned2024-04-26T21:18:27Z
dc.date.available2024-04-26T21:18:27Z
dc.date.issued2014-04-18
dc.description.abstractBackground: Several bacterial plant pathogens colonize their hosts through the secretion of effector proteins by a Type III protein secretion system (T3SS). The role of T3SS in bacterial pathogenesis is well established but whether this system is involved in multicellular processes, such as bacterial biofilm formation has not been elucidated. Here, the phytopathogen Xanthomonas citri subsp. citri (X. citri) was used as a model to gain further insights about the role of the T3SS in biofilm formation. Results: The capacity of biofilm formation of different X. citri T3SS mutants was compared to the wild type strain and it was observed that this secretion system was necessary for this process. Moreover, the T3SS mutants adhered proficiently to leaf surfaces but were impaired in leaf-associated growth. A proteomic study of biofilm cells showed that the lack of the T3SS causes changes in the expression of proteins involved in metabolic processes, energy generation, exopolysaccharide (EPS) production and bacterial motility as well as outer membrane proteins. Furthermore, EPS production and bacterial motility were also altered in the T3SS mutants. Conclusions: Our results indicate a novel role for T3SS in X. citri in the modulation of biofilm formation. Since this process increases X. citri virulence, this study reveals new functions of T3SS in pathogenesis.
dc.description.filFil: Zimaro, Tamara. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Sgro, Germán Gustavo. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Garofalo, Cecilia Graciela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ficarra, Florencia Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gottig, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Thomas, Ludivine. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.filFil: Marondedze, Claudius. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.filFil: Gehring, Chris. King Abdullah University of Science and Technology. Biological and Environmental Science and Engineering Division; Saudi Arabia.
dc.description.sponsorshipAgencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación (Agencia I+D+i): PICT2010-1507, PICT2010-0300
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (CONICET): PIP2010-2012
dc.description.sponsorshipFundación Josefina Prats
dc.description.versionpeerreviewed
dc.format.extent1-15
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/2133/26958
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.publisherversionhttps://doi.org/10.1186/1471-2180-14-96
dc.relation.publisherversionhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-14-96
dc.rightsopenAccess
dc.rights.holderZimaro, Tamara
dc.rights.holderSgro, Germán Gustavo
dc.rights.holderGarofalo, Cecilia Graciela
dc.rights.holderFicarra, Florencia Andrea
dc.rights.holderOttado, Jorgelina
dc.rights.holderGottig, Natalia
dc.rights.holderThomas, Ludivine
dc.rights.holderMarondedze, Claudius
dc.rights.holderGehring, Chris
dc.rights.holderUniversidad Nacional de Rosario
dc.rights.textAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectXanthomonas citri subsp. citri
dc.subjectBiofilm
dc.subjectT3SS
dc.subjectProteomics
dc.titleThe type III protein secretion system contributes to Xanthomonas citri subsp. citri biofilm formation
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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