Tomato second cycle hybrids as a source of genetic variability for fruit quality traits
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Brazilian Association of Plant Breeding
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The objective of this study was to investigate the phenotypic and molecular
variability in a F2 generation derived from a SCH (Second Cycle Hybrid)
in order to detect QTLs for some fruit traits of tomato. Genome coverage at
different levels was achieved by three types of molecular markers (polypeptides,
sequence-related amplified polymorphism-SRAP and amplified restriction fragment
polymorphism - AFLP). Different degrees of polymorphism were detected
by SRAP and AFLP at the DNA structure level and also by polypeptides at the DNA
expression level. The first two markers, associated with phenotypic variation,
detected QTLs involved in important agronomic traits such as fruit shelf life,
soluble solids content, pH, and titratable acidity. New gene blocks originated
by recombination during the first cycle of crossing were detected. This study
confirmed that the observed phenotypic differences represent a new gene rearrangement and that these new gene blocks are responsible for the presence of
the genetic variability detected for these traits.
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