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Development of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation steps

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dc.citation.titleMicrochemical Journal
dc.citation.volume133
dc.creatorLozano, Valeria Antonella
dc.creatorPrivitera, María Laura
dc.date.accessioned2017-12-04T21:03:04Z
dc.date.available2017-12-04T21:03:04Z
dc.date.issued2017-07
dc.descriptionA new strategy for the determination of riboflavin (RF, or vitamin B2) in human urine samples has been developed, combining excitation–emission fluorescence matrix (EEFM) data in standard addition mode and second–order chemometric analysis. The method is simple, fast and eco–friendly because it complies with the green analytical chemistry principles, avoiding the need of previous clean up and separation steps that consume high amounts of organic solvents. Successful results were obtained by different chemometric algorithms, namely parallel factor analysis (PARAFAC), unfolded partial least–squares/residual bilinearization (U–PLS/RBL) and multidimensional partial least–squares/residual bilinearization (N–PLS/RBL), all in the modified standard addition. These algorithms allowed us to achieve selectivity in a system, which requires standard addition and shows a significant background spectral overlapping with the studied vitamin. The quality of the proposed strategy was evidenced on the basis of the analytical recoveries from urine samples spiked with RF. The detection limits achieved in urine samples are encouraging compared to those obtained using chromatographic approaches. The relative prediction errors were lower than 5.6 %.es
dc.description.filFil: Lozano, Valeria Antonella. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR–CONICET); Argentina
dc.description.filFil: Privitera, María Laura. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario (IQUIR–CONICET); Argentina
dc.description.sponsorshipUniversidad Nacional de Rosario (Proyecto BIO 374)es
dc.description.sponsorshipConsejo Nacional de Investigaciones Científicas y Técnicas (Proyecto PIP 0163)es
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (Proyecto PICT 2013–0136)es
dc.formatapplication/pdf
dc.identifier.issn0026-265X
dc.identifier.urihttp://hdl.handle.net/2133/9737
dc.language.isoenges
dc.publisherElsevieres
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0026265X16303721es
dc.relation.publisherversionhttps://doi.org/10.1016/j.microc.2017.02.033es
dc.rightsopenAccesses
dc.rights.holderElsevieres
dc.rights.holderPrivitera, María L.es
dc.rights.holderLozano, Valeria A.es
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ar/*
dc.subjectHuman urine sampleses
dc.subjectGreen analytical chemistryes
dc.subjectSecond–order standard additiones
dc.subjectFluorescencees
dc.subjectChemometricses
dc.subjectRiboflavines
dc.titleDevelopment of a second–order standard addition fluorescence method for the direct determination of riboflavin in human urine samples without previous clean up and separation stepses

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FBIOyF - Departamento de Química Analítica - Artículos