Disentangling the regulatory response of Agrobacterium tumefaciens CHLDO to glyphosate for engineering whole-cell phosphonate biosensors

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dc.citation.titleACS Synthetic Biology
dc.citation.volume13
dc.contributor.orcidhttps://orcid.org/0000-0002-9313-7481
dc.creatorMasotti, Fiorella
dc.creatorKrink, Nicolas
dc.creatorLencina, Nicolas
dc.creatorGottig, Natalia
dc.creatorOttado, Jorgelina
dc.creatorNikel, Pablo I.
dc.date.accessioned2025-03-06T17:10:10Z
dc.date.available2025-03-06T17:10:10Z
dc.date.issued2024-09-30
dc.description.abstractPhosphonates (PHTs), organic compounds with a stable C−P bond, are widely distributed in nature. Glyphosate (GP), a synthetic PHT, is extensively used in agriculture and has been linked to various human health issues and environmental damage. Given the prevalence of GP, developing cost-effective, onsite methods for GP detection is key for assessing pollution and reducing exposure risks. We adopted Agrobacterium tumefaciens CHLDO, a natural GP degrader, as a host and the source of genetic parts for constructing PHT biosensors. In this bacterial species, the phn gene cluster, encoding the C−P lyase pathway, is regulated by the PhnF transcriptional repressor. We selected the phnG promoter, which displays a dose-dependent response to GP, to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cell biosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.
dc.description.filFil: Masotti, Fiorella. Universidad Nacional de Rosario. Facultas de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Krink, Nicolas. Technical University of Denmark. The Novo Nordisk Foundation Center for Biosustainability; Denmark.
dc.description.filFil: Lencina, Nicolas. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Gottig, Natalia. Instituto de Procesos Biotecnológicos y Químicos Rosario (IPROBYQ-CONICET); Argentina.
dc.description.filFil: Ottado, Jorgelina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina.
dc.description.filFil: Nikel, Pablo I. Technical University of Denmark. The Novo Nordisk Foundation Center for Biosustainability; Denmark.
dc.description.sponsorshipAgencia Nacional de Promoción Científica y Tecnológica (ANPCYT): 2020-0838
dc.description.sponsorshipMinisterio de Ciencia, Tecnología e Innovación (MINCYT): B19-CONVE-2021-78800688-APNSSCI#MCT
dc.description.sponsorshipThe Novo Nordisk Foundation: NNF10CC1016517, NNF18CC0033664,NNF23OC0083631
dc.description.versionpeerreviewed
dc.format.extent3430- 3445
dc.identifier.citationMasotti, F., Krink, N., Lencina, N., Gottig, N., Ottado, J., & Nikel, P. I. (2024). Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors. ACS synthetic biology, 13(10), 3430–3445. https://doi.org/10.1021/acssynbio.4c00497
dc.identifier.issn2161-5063
dc.identifier.urihttps://hdl.handle.net/2133/29008
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.publisherversionhttps://pubs.acs.org/doi/epdf/10.1021/acssynbio.4c00497?ref=article_openPDF
dc.relation.publisherversionhttps://doi.org/10.1021/acssynbio.4c00497
dc.rightsopenAccess
dc.rights.holderMasotti, Fiorella
dc.rights.holderKrink, Nicolas
dc.rights.holderLencina, Nicolas
dc.rights.holderGottig, Natalia
dc.rights.holderOttado, Jorgelina
dc.rights.holderNikel, Pablo I.
dc.rights.textAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectGlyphosate
dc.subjectWhole-cell biosensor
dc.subjectSynthetic biology
dc.subjectAgrobacterium
dc.subjectMetabolic engineering
dc.subjectBiodegradation
dc.titleDisentangling the regulatory response of Agrobacterium tumefaciens CHLDO to glyphosate for engineering whole-cell phosphonate biosensors
dc.typearticulo
dc.type.collectionarticulo
dc.type.versionpublishedVersion

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