Identification of key sequence features required for microRNA biogenesis in plants
dc.citation.title | Nature Communications | |
dc.citation.volume | 11 | |
dc.contributor.orcid | http://orcid.org/0000-0003-4316-4518 | |
dc.contributor.orcid | http://orcid.org/0000-0001-5350-514X | |
dc.contributor.orcid | http://orcid.org/0000-0003-2229-6853 | |
dc.contributor.orcid | http://orcid.org/0000-0002-1156-6662 | |
dc.contributor.orcid | http://orcid.org/0000-0002-2810-3533 | |
dc.contributor.orcid | http://orcid.org/0000-0002-2161-7910 | |
dc.contributor.orcid | http://orcid.org/0000-0002-9798-459X | |
dc.contributor.orcid | http://orcid.org/0000-0003-3940-067X | |
dc.contributor.orcid | http://orcid.org/0000-0001-5493-8537 | |
dc.contributor.orcid | http://orcid.org/0000-0001-7996-5224 | |
dc.creator | Rojas, Arantxa María Larisa | |
dc.creator | Drusin, Salvador Iván | |
dc.creator | Chorostecki, Uciel Pablo | |
dc.creator | Mateos, Julieta L. | |
dc.creator | Moro, Belén | |
dc.creator | Bologna, Nicolás G. | |
dc.creator | Bresso, Edgardo Gabriel | |
dc.creator | Schapire, Arnaldo L. | |
dc.creator | Rasia, Rodolfo M. | |
dc.creator | Moreno, Diego M. | |
dc.creator | Palatnik, Javier F. | |
dc.date.accessioned | 2024-08-29T16:57:52Z | |
dc.date.available | 2024-08-29T16:57:52Z | |
dc.date.issued | 2020-10-21 | |
dc.description.abstract | MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis. | |
dc.description.fil | Fil: Rojas, Arantxa María Larisa. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Drusin, Salvador Iván. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Drusin, Salvador Iván. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química-Física. Área Física; Argentina. | |
dc.description.fil | Fil: Chorostecki, Uciel Pablo. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Chorostecki, Uciel Pablo. Barcelona Supercomputing Centre (BSC-CNS); Spain. | |
dc.description.fil | Fil: Chorostecki, Uciel Pablo. Institute for Research in Biomedicine (IRB). The Barcelona Institute of Science and Technology; Spain. | |
dc.description.fil | Fil: Mateos, Julieta L. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Mateos, Julieta L. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET); Argentina. | |
dc.description.fil | Fil: Moro, Belén. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Moro, Belén. Centre for Research in Agricultural Genomics (CRAG); Spain. | |
dc.description.fil | Fil: Bologna, Nicolás G. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Bologna, Nicolás G. Centre for Research in Agricultural Genomics (CRAG); Spain. | |
dc.description.fil | Fil: Bresso, Edgardo Gabriel. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Schapire, Arnaldo L. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Rasia, Rodolfo M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Área Biofísica; Argentina. | |
dc.description.fil | Fil: Moreno, Diego M. Instituto de Química de Rosario (IQUIR-CONICET); Argentina. | |
dc.description.fil | Fil: Moreno, Diego M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Departamento de Química-Física. Área Química General e Inorgánica; Argentina. | |
dc.description.fil | Fil: Palatnik, Javier F. Universidad Nacional de Rosario. Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET); Argentina. | |
dc.description.fil | Fil: Palatnik, Javier F. Universidad Nacional de Rosario. Centro de Estudios Interdisciplinarios; Argentina. | |
dc.description.sponsorship | Ministerio de Ciencia, Tecnología e Innovación: PICT-2015-3557 | |
dc.description.sponsorship | International Centre For Genetic Engineering And Biotechnology (ICGEB CRP/ARG17-01) | |
dc.format.extent | 1-11 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://hdl.handle.net/2133/27670 | |
dc.language.iso | en | |
dc.publisher | Nature Research | |
dc.relation.publisherversion | https://doi.org/10.1038/s41467-020-19129-6 | |
dc.relation.publisherversion | https://www.nature.com/articles/s41467-020-19129-6 | |
dc.rights | openAccess | |
dc.rights.holder | Rojas, Arantxa María Larisa | |
dc.rights.holder | Drusin, Salvador Iván | |
dc.rights.holder | Chorostecki, Uciel Pablo | |
dc.rights.holder | Mateos, Julieta L. | |
dc.rights.holder | Moro, Belén | |
dc.rights.holder | Bologna, Nicolás G. | |
dc.rights.holder | Bresso, Edgardo Gabriel | |
dc.rights.holder | Schapire, Arnaldo L. | |
dc.rights.holder | Rasia, Rodolfo M. | |
dc.rights.holder | Moreno, Diego M. | |
dc.rights.holder | Palatnik, Javier F. | |
dc.rights.holder | Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas | |
dc.rights.text | Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Arabidopsis | |
dc.subject | Ribonucleasa III | |
dc.subject | MicroARNs | |
dc.subject | Magnoliopsida | |
dc.subject | Nucleic acid conformation | |
dc.title | Identification of key sequence features required for microRNA biogenesis in plants | |
dc.type | articulo | |
dc.type.collection | articulo | |
dc.type.version | publishedVersion |
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