Evaluation of SRAP markers for mapping of Pisum sativum L.
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Brazilian Society of Plant Breeding
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Linkage maps have become important tools for genetic studies. With
the aim of evaluating the SRAP (sequence-related amplified polymorphism)
technique for linkage mapping in Pisum sativum L., a F2
mapping population
derived from an initial cross between cvs. DDR11 and Zav25 was generated. A
total of 25 SRAP primer combinations were evaluated in 45 F2
plants and both
parental lines, generating 208 polymorphic bands/markers. The markers were
analyzed by the chi-square goodness-of-fit test to check the expected Mendelian
segregation ratio. The resulting linkage map consists of 112 genetic markers
distributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length of
the LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers.
The linkage map developed in this study indicates that the SRAP marker system
could be applied to mapping studies of pea.